Atrophin 1 Protein (ATN1) (AA 1-1175) (Strep Tag)

Produktnummer ABIN3131438

Produktdetails

Target: Atrophin 1 (ATN1)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1175

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses Atrophin 1 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MKTRQNKDSM SMRSGRKKEA PGPREELRSR GRASPGGVST SSSDGKAEKS RQTAKKARIE EPSAPKASKQ GRSEEISESE SEETSAPKKT KTEQELPRPQ SPSDLDSLDG RSINDDGSSD PRDIDQDNRS TSPSIYSPGS VENDSDSSSG LSQGPARPYH PPPLFPPSPP PPDSTPRQPE SGFEPHPSVP PTGYHAPMEP PTSRLFQGPP PGAPPTHPQL YPGNASGGVL SGPPMGPKGG AAASSVGAPS GGKQHPPPTT PIPISSSGAS GAPPAKPPSA PVGGGSLPSA PPPASFPHVT PNLPPPPALR PLNNASASPP GMGAQPIPGH LPSPHAMGQG MSGLPPGPEK GPTLAPSPHP LPPASSSAPG PPMRYPYSSS SSSAAASSSS SSSSASQYPA SQALPSYPHS FPPPTSMSVS NQPPKYTQPS LPSQAVWSQG PPPPPPYGRL LANNNTHPGP FPPTGGQSTA HPAAPTHHHH QQQPQQQHHH GNSGPPPPGA YPHPLESSNS HHAHPYNMSP SLGSLRPYPP GPAHLPPPHG QVSYNQAGPN GPPVSSSNSS GSSSQASYSC SHPSSSQGPQ GASYPFPPVP PVTTSSATLS TVIATVASSP AGYKTASPPG PPQYSKRAPS PGSYKTATPP GYKPGSPPSF RTGTPPGYRG TSPPAGPGTF KPGSPTVGPG PLPPAGPSSL SSLPPPPAAP TTGPPLTATQ IKQEPAEEYE PPESPVPPAR SPSPPPKVVD VPSHASQSAR FNKHLDRGFN SCARSDLYFV PLEGSKLAKK RADLVEKVRR EAEQRAREEK EREREREREK EREREKEREL ERSVKLAQEG RAPVECPSLG PVPHRPPFEP GSAVATVPPY LGPDTPALRT LSEYARPHVM SPGNRNHPFY VPLGAVDPGL LGYNVPALYS SDPAARERER EARERDLRDR LKPGFEVKPS ELEPLHGVPG PGLDPFPRHG GLALQPGPPG LHPFPFHPSL GPLERERLAL AAGPALRPDM SYAERLAAER QHAERVAALG NDPLARLQML NVTPHHHQHS HIHSHLHLHQ QDAIHAASAS VHPLIDPLAS GSHLTRIPYP AGTLPNPLLP HPLHENEVLR HQLFAAPYRD LPASLSAPMS AAHQLQAMHA QSAELQRLAL EQQQWLHAHH PLHSVPLPAQ EDYYSHLKKE SDKPL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Atrophin 1 (ATN1)

Andere Bezeichnung: Atn1 (ATN1 Produkte)

Synonyme: B37 Protein, D12S755E Protein, DRPLA Protein, HRS Protein, NOD Protein, Atr1 Protein, Drpla Protein, atrophin-1 Protein, RERE Protein, ATN1 Protein, atrophin 1 Protein, ATN1 Protein, Atn1 Protein

Hintergrund: Atrophin-1 (Dentatorubral-pallidoluysian atrophy protein homolog),FUNCTION: Transcriptional corepressor. Corepressor of MTG8 transcriptional repression. Has some intrinsic repression activity which is independent of the number of the poly-Q repeats (By similarity). Recruits NR2E1 to repress transcription. Promotes vascular smooth cell (VSMC) migration and orientation. {ECO:0000250|UniProtKB:P54259, ECO:0000269|PubMed:16702404, ECO:0000269|PubMed:19131340}.

Molekulargewicht: 123.7 kDa

UniProt: O35126