Arx Protein (AA 1-564) (Strep Tag)

Produktnummer ABIN3131427

Produktdetails

Target: Arx (ARX) (Aristaless Related Homeobox (ARX))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-564

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses Arx Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MSNQYQEEGC SERPECKSKS PTLLSSYCID SILGRRSPCK MRLLGAAQSL PAPLASRADQ EKAMQGSPKS SSAPFEAELH LPPKLRRLYG PGGGRLLQGA AAAAAAAAAA AAAATATGTA GPRGEVPPPP PPAARPGERQ DSAGAVAAAA AAAAWDTLKI SQAPQVSISR SKSYRENGAP FVPPPPALDE LSGPGGVAHP EERLSAASGP GSAPAAGGGT GAEDDEEELL EDEEDEEEEE ELLEDDDEEL LEDDARALLK EPRRCSVATT GTVAAAAAAA AAAVATEGGE LSPKEELLLH PEDAEGKDGE DSVCLSAGSD SEEGLLKRKQ RRYRTTFTSY QLEELERAFQ KTHYPDVFTR EELAMRLDLT EARVQVWFQN RRAKWRKREK AGAQTHPPGL PFPGPLSATH PLSPYLDASP FPPHHPALDS AWTAAAAAAA AAFPSLPPPP GSASLPPSGA PLGLSTFLGA AVFRHPAFIS PAFGRLFSTM APLTSASTAA ALLRQPTPAV EGAVASGALA DPATAAADRR ASSIAALRLK AKEHAAQLTQ LNILPGTSTG KEVC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Arx (ARX) (Aristaless Related Homeobox (ARX))

Andere Bezeichnung: Arx (ARX Produkte)

Synonyme: CT121 Protein, EIEE1 Protein, ISSX Protein, MRX29 Protein, MRX32 Protein, MRX33 Protein, MRX36 Protein, MRX38 Protein, MRX43 Protein, MRX54 Protein, MRX76 Protein, MRX87 Protein, MRXS1 Protein, PRTS Protein, phox2a Protein, RGD1562672 Protein, arx Protein, arx-a Protein, arx-b Protein, arx2 Protein, ct121 Protein, issx Protein, mrx29 Protein, mrx32 Protein, mrx33 Protein, mrx36 Protein, mrx38 Protein, mrx43 Protein, mrx54 Protein, mrx76 Protein, mrx87 Protein, mrxs1 Protein, prt5 Protein, xARX Protein, aristaless related homeobox Protein, aristaless related homeobox a Protein, aristaless related homeobox L homeolog Protein, Arx Protein, ARX Protein, arxa Protein, arx.L Protein

Hintergrund: Homeobox protein ARX (Aristaless-related homeobox),FUNCTION: Transcription factor (By similarity). Binds to specific sequence motif 5'-TAATTA-3' in regulatory elements of target genes, such as histone demethylase KDM5C (By similarity). Positively modulates transcription of KDM5C (PubMed:31691806). Activates expression of KDM5C synergistically with histone lysine demethylase PHF8 and perhaps in competition with transcription regulator ZNF711, synergy may be related to enrichment of histone H3K4me3 in regulatory elements (By similarity). Required for normal brain development (PubMed:12379852). Plays a role in neuronal proliferation, interneuronal migration and differentiation in the embryonic forebrain (PubMed:12379852, PubMed:31691806). May also be involved in axonal guidance in the floor plate (PubMed:9256348). {ECO:0000250|UniProtKB:Q96QS3, ECO:0000269|PubMed:12379852, ECO:0000269|PubMed:31691806, ECO:0000269|PubMed:9256348}.

Molekulargewicht: 58.5 kDa

UniProt: O35085