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OTP Protein (AA 1-325) (His tag)

Crystallography grade OTP Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3131392
  • Target Alle OTP Proteine anzeigen
    OTP (Orthopedia Homeobox (OTP))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-325
    Spezies
    • 3
    • 1
    Maus
    Quelle
    • 2
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses OTP Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MLSHADLLDA RLGMKDAAEL LGHREAVKCR LGVGGSDPGG HPGDLAPNSD PVEGATLLPG EDITTVGSTP ASLAVSAKDP DKQPGPQGGP NPSQAGQQQG QQKQKRHRTR FTPAQLNELE RSFAKTHYPD IFMREELALR IGLTESRVQV WFQNRRAKWK KRKKTTNVFR APGTLLPTPG LPQFPSAAAA AAAAMGDSLC SFHANDTRWA AAAMPGVSQL PLPPALGRQQ AMAQSLSQCS LAAGPPPNSM GLSNSLAGSN GAGLQSHLYQ PAFPGMVPAS LPGPSNVSGS PQLCSSPDSS DVWRGTSIAS LRRKALEHTV SMSFT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Otp Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product OTP Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    OTP (Orthopedia Homeobox (OTP))
    Andere Bezeichnung
    Otp (OTP Produkte)
    Synonyme
    OTP Protein, otp Protein, BcDNA:RE58095 Protein, CG10036 Protein, CG2965 Protein, Dmel\\CG10036 Protein, Otp Protein, W26 Protein, bk24 Protein, w26 Protein, cb977 Protein, otp1 Protein, orthopedia homeobox Protein, orthopedia Protein, orthopedia homeobox a Protein, orthopedia homeobox b Protein, OTP Protein, Otp Protein, otp Protein, otpa Protein, otpb Protein
    Hintergrund
    Involved in the specification of hypothalamic neuroendocrine cells. Specifically required for the specification of diencephalic dopaminergic neurons of the A11 group. {ECO:0000269|PubMed:10557207, ECO:0000269|PubMed:11071765, ECO:0000269|PubMed:17481897, ECO:0000269|PubMed:7913821}.
    Molekulargewicht
    35.1 kDa Including tag.
    UniProt
    O09113
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