TCOF1 Protein (AA 1-1320) (Strep Tag)

Produktnummer ABIN3131310

Produktdetails

Target: TCOF1 (Treacher Collins-Franceschetti Syndrome 1 (TCOF1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1320

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TCOF1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAEARKRREL LPLIYHHLLQ AGYVRAAREV KEQSGQKSFL TQPVTLLDIY THWQQTSELG QKQKAEDDET LQAKKSRVSD PVSSSESSDQ EKEEEAATER AKATPRPTPV NSATAALPSK VKEKGKTKTA NKTVNSVSHP GSGKTVVHLL SGKSPKKSAE PLANTVLASE TEEEGNAQAL GPTAKSGTVS AGQGSSSSED SSISSDETDV EVKSPAKPAQ AKASAAPAKD PPARTAPGPT KLGNVAPTPA KPARAAAAAA AAAVAAAAAA AAEESESSEE DSDSEDEAPA GLPSQVKASG KGPHVRADSV SAKGISGKGP ILATPGKTGP AATQAKAERP EKDSETSSED DSDSEDEMPV TVNTPQARTS GKSPRARGTS APAKESSQKG APAVTPGKAR PVAAQAGKPE AKSSEESESD SGETPAAATL TTSPAKVKPL GKSSQVRPVS TVTPGSSGKG ANLPCPGKVG SAALRVQMVK KEDVSESSSA ELDSDGPGSP AKAKASLALP QKVRPVATQV KTDRGKGHSG SSEESSDSEE EAAPAASAAQ AKPALEKQMK ASSRKGTPAS ATGASTSSHC KAGAVTSSAS LSSPALAKGT QRSDVDSSSE SESEGAAPST PRVQGKSGGK GLQGKAALGQ GVAPVHTQKT GPSVKAMAQE DSESLEEDSS SEEEDETPAQ ATPLGRLPQA KANPPPTKTP PASASGKAVA APTKGKPPVP NSTVSARGQR SVPAAGKAGA PATQAQKGPV AGTGEDSESS SKEESDSEEE TPAQIKPVGK TSQVRAASAP AKESPKKGAH PGTPGKTGSS ATQAQPGKTE DSDSSSEESD SDTEMPSAQA IKSPPVSVNR NSSPAVPAPT PEGVQAVNTT KKASGTTAQS SSSESEDGDE DLIPATQPST YALRTSVTTP AALSRAASQP SKSEQSSRMP KGKKAKAAAS AQTSSAVETL PMMPPQSAPI QPKATNKLGK SKLPEKQQLA PGYPKAPRSS EDSSDTSSED EEDAKRPQMP KSAHRLDPDP SQKETVVEET PTESSEDEMV APSQSLLSGY MTPGLTVANS QASKATPRPD SNSLASSAPA TKDNPDGKQK SKSQHAADTA LPKTGRKEAS SGSTPQKPKK LKKSTSSSPA PTQTLPNSIT QRLLEQAWPL SEAQVQASVV KVLTELLEQE RLKATEAIKE SGKKSQKRKL SGDLEAGAPK NKKKKEQPVP RASAVSPEKA PMTSKAKSKL DKGSAGGKGK GSPGPQGAKE KPDGELLGIK LESGEQSDPK SKSKKKKSLK KKKDKEKKEK KKGKKSLAKD SASPIQKKKK KKKKSAEPAV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TCOF1 (Treacher Collins-Franceschetti Syndrome 1 (TCOF1))

Andere Bezeichnung: Tcof1 (TCOF1 Produkte)

Synonyme: TCOF1 Protein, AA408847 Protein, AW209012 Protein, treacle Protein, MFD1 Protein, TCS1 Protein, treacle ribosome biogenesis factor 1 Protein, Tcof1 Protein, TCOF1 Protein

Hintergrund: Treacle protein (Treacher Collins syndrome protein homolog),FUNCTION: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. {ECO:0000250|UniProtKB:Q13428}.

Molekulargewicht: 135.0 kDa

UniProt: O08784