Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

ONECUT1 Protein (AA 1-465) (His tag)

Crystallography grade ONECUT1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
Produktnummer ABIN3131306
  • Target Alle ONECUT1 Proteine anzeigen
    ONECUT1 (One Cut Homeobox 1 (ONECUT1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-465
    Spezies
    • 1
    • 1
    Maus
    Quelle
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses ONECUT1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Crystallization (Crys), SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MNAQLTMEAI GELHGVSHEP VPAPADLLGG SPHARSSVGH RGSHLPPAHP RSMGMASLLD GGSGGSDYHH HHRAPEHSLA GPLHPTMTMA CETPPGMSMP TTYTTLTPLQ PLPPISTVSD KFPHHHHHHH HHHHPHHHQR LAGNVSGSFT LMRDERGLAS MNNLYTPYHK DVAGMGQSLS PLSGSGLGSI HNSQQGLPHY AHPGAAMPTD KMLTPNGFEA HHPAMLGRHG EQHLTPTSAG MVPINGLPPH HPHAHLNAQG HGQLLGTARE PNPSVTGAQV SNGSNSGQME EINTKEVAQR ITTELKRYSI PQAIFAQRVL CRSQGTLSDL LRNPKPWSKL KSGRETFRRM WKWLQEPEFQ RMSALRLAAC KRKEQEHGKD RGNTPKKPRL VFTDVQRRTL HAIFKENKRP SKELQITISQ QLGLELSTVS NFFMNARRRS LDKWQDEGGS NSGSSSSSSS TCTKA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Onecut1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ONECUT1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ONECUT1 (One Cut Homeobox 1 (ONECUT1))
    Andere Bezeichnung
    Onecut1 (ONECUT1 Produkte)
    Synonyme
    D9Ertd423e Protein, HNF-6 Protein, Hfh12 Protein, Hnf6 Protein, Hnf6b Protein, OC-1 Protein, Oc1 Protein, onecut Protein, onecut1 Protein, id:ibd5005 Protein, HNF6 Protein, HNF6A Protein, HNF-6a Protein, oc1 Protein, onecut1l Protein, zgc:65904 Protein, zgc:77450 Protein, one cut domain, family member 1 Protein, one cut homeobox 1 Protein, one cut domain, family member, like Protein, Onecut1 Protein, onecutl Protein, ONECUT1 Protein, onecut1 Protein
    Hintergrund
    Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. Stimulates the expression of Onecut3 in the developing endoderm. {ECO:0000269|PubMed:15381696}.
    Molekulargewicht
    51.9 kDa Including tag.
    UniProt
    O08755
Sie sind hier:
Kundenservice