OAZ2 Protein (AA 1-189) (Strep Tag)

Produktnummer ABIN3131262

Produktdetails

Target: OAZ2 (Ornithine Decarboxylase Antizyme 2 (OAZ2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-189

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses OAZ2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MINTQDSSIL PLSKCPQLQC CRHIVPGPLW CSDAPHPLSK IPGGRGGGRD PSLSALIYKD EKLTVTQDLP VNDGKPHIVH FQYEVTEVKV SSWDAVLSSQ SLFVEIPDGL LADGSKEGLL ALLEFAEEKM KVNYVFICFR KGREDRAPLL KTFSFLGFEI VRPGHPCVPS RPDVMFMVYP LDQNLSDED
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: OAZ2 (Ornithine Decarboxylase Antizyme 2 (OAZ2))

Andere Bezeichnung: Oaz2 (OAZ2 Produkte)

Synonyme: AZ2 Protein, AZ-2 Protein, Oaz2-ps Protein, Sez15 Protein, AZL Protein, oaz2 Protein, wu:fa14f04 Protein, zgc:63816 Protein, RGD1562933 Protein, si:dkey-275p19.6 Protein, ornithine decarboxylase antizyme 2 Protein, ornithine decarboxylase antizyme 1b Protein, ornithine decarboxylase antizyme 2 S homeolog Protein, ornithine decarboxylase antizyme 2a Protein, OAZ2 Protein, Oaz2 Protein, oaz1b Protein, oaz2.S Protein, Tsp_12111 Protein, oaz2 Protein, oaz2a Protein

Hintergrund: Ornithine decarboxylase antizyme 2 (AZ2) (ODC-Az 2) (Seizure-related protein 15),FUNCTION: Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis and uptake in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimers. Does not target the ODC monomers for degradation, which allows a protein synthesis-independent restoration of ODC activity (PubMed:16916800, PubMed:18508777, PubMed:24967154). Involved in the translocation of AZIN2 from ER-Golgi intermediate compartment (ERGIC) to the cytosol (PubMed:19449338). {ECO:0000269|PubMed:16916800, ECO:0000269|PubMed:18508777, ECO:0000269|PubMed:19449338, ECO:0000269|PubMed:24967154}.

Molekulargewicht: 21.0 kDa

UniProt: O08608