IRF2BP2 Protein (AA 2-570) (His tag)

Produktnummer ABIN3131141

Produktdetails

Target: IRF2BP2 (Interferon Regulatory Factor 2 Binding Protein 2 (IRF2BP2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-570

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses IRF2BP2 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: AAAVAVAAAS RRQSCYLCDL PRMPWAMIWD FTEPVCRGCV NYEGADRVEF VIETARQLKR AHGCFPEGRS PTGAQPAAAK PPPLSAKDLL LQPPPQLGHA GAEAARAQAM ERYPLAPDRA PRLASDFSTR AGAGLPQSAA QQSAPANGIL VPNGFSKLEE PPELNRQSPN PRRAHAVPPT LVPLVNGSAA LGLSGRAAAT LAAVSGTPGL GAQPAELGTH KRPASVSSAA AEHEAREPSK EKAQPAHRSP ADSLSSAAGA SELSAEGAGK GRAPGEQDWA SRPKTVRDTL LALHQHGHSG PFESKFKKEP ALTAAGRLLG FEANGANGSK AVARTARKRK PSPEPEGEVG PPKINGETQP WLSTSTEGLK IPITPTSSFV SPPPPTASPH SNRTTPPEAA QNGQSPMAAL ILVADNAGGS HASKDATQVH STTRRNSSSP PSPSSMNQRR LGPREVGGQA TGSTGGLEPV HPASLPDSSL AASAPLCCTL CHERLEDTHF VQCPSVPSHK FCFPCSRQSI KQQGASGEVY CPSGEKCPLV GSNVPWAFMQ GEIATILAGD VKVKKERDS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Irf2bp2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: IRF2BP2 (Interferon Regulatory Factor 2 Binding Protein 2 (IRF2BP2))

Andere Bezeichnung: Irf2bp2 (IRF2BP2 Produkte)

Synonyme: E130305N23Rik Protein, IRF-2BP2 Protein, 5n852 Protein, irf2bp2-A Protein, IRF2BP2 Protein, fj35c12 Protein, irf2bp2b Protein, wu:fj35c12 Protein, zgc:56251 Protein, IRF-2-binding protein 2-B Protein, IRF-2BP2-B Protein, irf2bp2 Protein, irf2bp2a Protein, wu:fa12b04 Protein, wu:fb73g01 Protein, zgc:63864 Protein, interferon regulatory factor 2 binding protein 2 Protein, interferon regulatory factor 2 binding protein 2 L homeolog Protein, interferon regulatory factor 2 binding protein 2a Protein, interferon regulatory factor 2 binding protein 2b Protein, Irf2bp2 Protein, IRF2BP2 Protein, irf2bp2.L Protein, irf2bp2a Protein, irf2bp2b Protein

Hintergrund: Acts as a transcriptional corepressor in a IRF2-dependent manner, this repression is not mediated by histone deacetylase activities. Represses the NFAT1-dependent transactivation of NFAT-responsive promoters. Acts as a coactivator of VEGFA expression in cardiac and skeletal muscle (By similarity). {ECO:0000250}.

Molekulargewicht: 60.1 kDa Including tag.

UniProt: E9Q1P8