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PTPRZ1 Protein (AA 1659-2312) (His tag)

Crystallography grade PTPRZ1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3131069
  • Target Alle PTPRZ1 Proteine anzeigen
    PTPRZ1 (Protein tyrosine Phosphatase, Receptor-Type, Z Polypeptide 1 (PTPRZ1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1659-2312
    Spezies
    • 2
    • 1
    Maus
    Quelle
    • 3
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses PTPRZ1 Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    WRKCFQTAHF YLEDNTSPRV ISTPPTPIFP ISDDIGAIPI KHFPKHVADL HASNGFTEEF ETLKEFYQEV QSCTADLGIT ADSSNHPDNK HKNRYVNIVA YDHSRVKLTQ LAEKDGKLTD YINANYVDGY NRPKAYIAAQ GPLKSTAEDF WRMIWEHNVE VIVMITNLVE KGRRKCDQYW PTDGSEEYGS FLVNQKSVQV LAYYTVRNFT LRNTKLKKGS QKGRSSGRLV TQYHYTQWPD MGVPEYSLPV LAFVRKAAQA KRHAVGPVVV HCSAGVGRTG TYIVLDSMLQ QIQHEGTVNI FGFLKHIRSQ RNYLVQTEEQ YVFIHDTLVE AILSKETEVP DSHIHSYVNT LLIPGPTGKT KLEKQFQLLS QSNILQSDYS TALKQCNREK NRTSSIIPVE RSRVGISSLS GEGTDYINAS YIMGYYQSNE FIITQHPLLH TIKDFWRMIW DHNAQLVVMI PDGQNMAEDE FVYWPNKDEP INCESFKVTL MSEEHKCLSN EEKLIVQDFI LEATQDDYVL EVRHFQCPKW PNPDSPISKT FELISIIKEE AANRDGPMIV HDEHGGVTAG TFCALTTLMH QLEKENAMDV YQVAKMINLM RPGVFTDIEQ YQFLYKVVLS LVSTRQEENP STSLDSNGAA LPDGNIAESL ESLV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ptprz1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product PTPRZ1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    PTPRZ1 (Protein tyrosine Phosphatase, Receptor-Type, Z Polypeptide 1 (PTPRZ1))
    Andere Bezeichnung
    Ptprz1 (PTPRZ1 Produkte)
    Synonyme
    HPTPZ Protein, HPTPzeta Protein, PTP-ZETA Protein, PTP18 Protein, PTPRZ Protein, PTPZ Protein, R-PTP-zeta-2 Protein, RPTPB Protein, RPTPbeta Protein, phosphacan Protein, Ptpz Protein, AI850339 Protein, DSD-1-PG Protein, PTPbeta Protein, PTPzeta Protein, Ptprz Protein, RPTPz Protein, Rptpbeta Protein, protein tyrosine phosphatase, receptor type Z1 Protein, protein tyrosine phosphatase, receptor type Z, polypeptide 1 Protein, PTPRZ1 Protein, Ptprz1 Protein
    Hintergrund
    Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades. {ECO:0000269|PubMed:12355066, ECO:0000269|PubMed:16513268, ECO:0000269|PubMed:21969550}.
    Molekulargewicht
    75.5 kDa Including tag.
    UniProt
    B9EKR1
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