PTPRB Protein (AA 23-1622) (His tag)

Produktnummer ABIN3131020

Produktdetails

Target: PTPRB (Protein tyrosine Phosphatase, Receptor Type, B (PTPRB))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-1622

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PTPRB Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: EQVKCNFTLL ESRVSSLSAS IQWRTFASPC NFSLIYSSDT SGPMWCHPIR IDNFTYGCNP KDLQAGTVYN FRIVSLDGEE STLVLQTDPL PPARFEVNRE KTASTTLQVR WTPSSGKVSW YEVQLFDHNN QKIQEVQVQE STTWSQYTFL NLTEGNSYKV AITAVSGEKR SFPVYINGST VPSPVKDLGI SPNPNSLLIS WSRGSGNVEQ YRLVLMDKGA IVQDTNVDRR DTSYAFHELT PGHLYNLTIV TMASGLQNSR WKLVRTAPME VSNLKVTNDG RLTSLNVKWQ KPPGDVDSYS ITLSHQGTIK ESKTLAPPVT ETQFKDLVPG RLYQVTISCI SGELSAEKSA AGRTVPEKVR NLVSYNEIWM KSFTVNWTPP AGDWEHYRIV LFNESLVLLN TTVGKEETHY ALDGLELIPG RQYEIEVIVE SGNLRNSERC QGRTVPLAVL QLRVKHANET SLGITWRAPL GEWEKYIISL MDRELLVIHK SLSKDAKEFT FTDLMPGRNY KATVTSMSGD LKQSSSIKGR TVPAQVTDLH VNNQGMTSSL FTNWTKALGD VEFYQVLLIH ENVVVKNESV SSDTSRYSFR ALKPGSLYSV VVTTVSGGIS SRQVVAEGRT VPSSVSGVTV NNSGRNDYLS VSWLPAPGEV DHYVVSLSHE GKVDQFLIIA KSVSECSFSS LTPGRLYNVT VTTKSGNYAS HSFTEERTVP DKVQGISVSN SARSDYLKVS WVHATGDFDH YEVTIKNRES FIQTKTIPKS ENECEFIELV PGRLYSVTVS TKSGQYEASE QGTGRTIPEP VKDLTLLNRS TEDLHVTWSR ANGDVDQYEV QLLFNDMKVF PHIHLVNTAT EYKFTALTPG RHYKILVLTI SGDVQQSAFI EGLTVPSTVK NIHISANGAT DRLMVTWSPG GGDVDSYVVS AFRQDEKVDS QTIPKHASEH TFHRLEAGAK YRIAIVSVSG SLRNQIDALG QTVPASVQGV VAANAYSSNS LTVSWQKALG VAERYDILLL NENGLLLSNV SEPATARQHK FEDLTPGKKY KMQILTVSGG LFSKESQAEG RTVPAAVTNL RITENSSRYL SFGWTASEGE LSWYNIFLYN PDRTLQERAQ VDPLVQSFSF QNLLQGRMYK MVIVTHSGEL SNESFIFGRT VPAAVNHLKG SHRNTTDSLW FSWSPASGDF DFYELILYNP NGTKKENWKE KDVTEWRFQG LVPGRKYTLY VVTHSGDLSN KVTGEGRTAP SPPSLLSFAD VANTSLAITW KGPPDWTDYN DFELQWFPGD ALTIFNPYSS RKSEGRIVYG LHPGRSYQFS VKTVSGDSWK TYSKPISGSV RTKPDKIQNL HCRPQNSTAI ACSWIPPDSD FDGYSIECRK MDTQEIEFSR KLEKEKSLLN IMMLVPHKRY LVSIKVQSAG MTSEVVEDST ITMIDRPPQP PPHIRVNEKD VLISKSSINF TVNCSWFSDT NGAVKYFAVV VREADSMDEL KPEQQHPLPS YLEYRHNASI RVYQTNYFAS KCAESPDSSS KSFNIKLGAE MDSLGGKCDP SQQKFCDGPL KPHTAYRISI RAFTQLFDED LKEFTKPLYS DTFFSMPITT ESEPLFGVIE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ptprb Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PTPRB (Protein tyrosine Phosphatase, Receptor Type, B (PTPRB))

Andere Bezeichnung: Ptprb (PTPRB Produkte)

Synonyme: PTPRB Protein, HPTP-BETA Protein, HPTPB Protein, PTPB Protein, R-PTP-BETA Protein, VEPTP Protein, 3230402H02Rik Protein, C130094E24 Protein, Ptpz Protein, Rptpb Protein, VE-PTP Protein, Veptp Protein, protein tyrosine phosphatase, receptor type B Protein, receptor-type tyrosine-protein phosphatase beta Protein, protein tyrosine phosphatase, receptor type, B Protein, PTPRB Protein, LOC100380677 Protein, Ptprb Protein

Hintergrund: Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin. {ECO:0000269|PubMed:10557082, ECO:0000269|PubMed:12234928, ECO:0000269|PubMed:16514057, ECO:0000269|PubMed:17360632, ECO:0000269|PubMed:19015309, ECO:0000269|PubMed:19451274}.

Molekulargewicht: 179.7 kDa Including tag.

UniProt: B2RU80