SKOR2 Protein (AA 1-1008) (Strep Tag)

Produktnummer ABIN3130932

Produktdetails

Target: SKOR2 (SKI Family Transcriptional Corepressor 2 (SKOR2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1008

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SKOR2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MASSPLPGPN DILLASPSSA FQPDALSQPR PGHANLKPNQ VGQVILYGIP IVSLVIDGQE RLCLAQISNT LLKNFSYNEI HNRRVALGIT CVQCTPVQLE ILRRAGAMPI SSRRCGMITK REAERLCKSF LGENRPPKLP DNFAFDVSHE CAWGCRGSFI PARYNSSRAK CIKCSYCNMY FSPNKFIFHS HRTPDAKYTQ PDAANFNSWR RHLKLTDKSP QDELVFAWED VKAMFNGGSR KRALPQPSAH PACHPLSSVK AAAVAAAAAV AGGGGLLGPH LLGAPPPPPP PPPLAELAGA PHAHHKRPRF DDDDDSLQEA AVVAAASLSA AAASLSVAAA TGGAGPGAGG PGGGCVAGVG VGASAGAGAA AGTKGPRSYP VIPVPSKGSF GGVLQKFPGC GGLFPHPYTF PAAAAAFGLC HKKEDAGTAA EALGGAGAGS AGAAPKAGLS GLFWPAGRKD AFYPPFCMFW PPRTPGGLPV PTYLQPPPQP PSALGCALGD SPALLRQAFL DLAEPGGAGG SAEAAPPPGQ PPPVVANGPG SGPPATGGTG ARDTLFESPP GGSGGDCSAG STPPAEQGVT SGTGSASSGA GSVGTRVPAP HHPHLLEGRK AGGGSYHHSS AFRPVGGKDD AESLAKLHGA SAGTPHSAPA HHHHHHHHPH HHHHHPPQPP SPLLLLQPQP DEPGSERHHP APPPPPPPPP LAPQPHHRGL LSPEGTSCSY PSEDSSEDEE DEEEEQEVDV EGHKPLEGEE EEDGRDPEDE EEEDEETRVL LGDSLVGGGR FLQGRGLSEK GSGRDRTTPA VGAFPLALNS SRLLQEDGKL GDSGGSDLPA PPPPPLAPQK ASSSGGSRPG SPVHHPSLEE EPSYKDNQKP KENNQVIIST KDDNFSDKNK GHGFFITDSD SSGDFWRERS GEHTQETNSP HSLKKDVENM GKEELQKVLF EQIDLRRRLE QEFQVLKGNT SFPVFNNFQD QMKRELAYRE EMVQQLQIIP YAASLIRKEK LGAHLSKS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SKOR2 (SKI Family Transcriptional Corepressor 2 (SKOR2))

Andere Bezeichnung: Skor2 (SKOR2 Produkte)

Synonyme: CORL2 Protein, FUSSEL18 Protein, Corl2 Protein, EG664805 Protein, Fussel18 Protein, Gm7348 Protein, SKI family transcriptional corepressor 2 Protein, SKOR2 Protein, Skor2 Protein

Hintergrund: SKI family transcriptional corepressor 2 (Fussel-18 homolog) (LBX1 corepressor 1-like protein) (Ladybird homeobox corepressor 1-like protein) (Transcriptional corepressor Corl2),FUNCTION: Acts as a TGF-beta antagonist in the nervous system (By similarity). Exhibits transcriptional repressor activity. {ECO:0000250, ECO:0000269|PubMed:18522874}.

Molekulargewicht: 105.6 kDa

UniProt: A7M7C7