Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

TTLL3 Protein (AA 1-927) (His tag)

Crystallography grade TTLL3 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3130890
  • Target Alle TTLL3 Proteine anzeigen
    TTLL3 (Tubulin tyrosine Ligase-Like Family, Member 3 (TTLL3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-927
    Spezies
    Maus
    Quelle
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses TTLL3 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MQGVSSALLL SAGQLGPGAA WYRQEGSSEC SWLRRSQPSE LRTNFSSRWP WPRNSESRRS ERLQWPGPAS AKPEVASCGD SRRDYSSLPA RHLSSARESS MPGALGTVNP QPVRTLVPPT LDEPLPDALR PPDDSLLLWR GLTKGPNHMG RLRNAKIHVE RAVKQKKIFM IHGRYPVIRC LLRQRGWVEK KMVHPPGTAL PAPQKDLDSS MLGDSDATED EDEEENEMFR ESQLLDLDGF LEFDDLDGIH ALMSRMVRNE TPYLIWTTRR DVLDCRFLSK DQMINHYARA GSFTTKVGLC LNLRNLPWFD EADADSFFPR CYRLGAEDDK KAFIEDFWLT AARNVLKLVV KLEEKSQSIS IQAREEEAPE DTQPKKQEKK LVTVSSDFVD EALSACQEHL SSIAHKDIDK DPNSPLYLSP DDWSQFLQRY YQIVHEGAEL RYLEVQVQRC EDILQQLQNV VPQLDMEGDR NIWIVKPGAK SRGRGIMCMN RLDEMLKLVD CNPMLMKDGK WIVQKYIERP LLIFGTKFDL RQWFLVTDWN PLTVWFYRDS YIRFSTQPFS LKNLDNSVHL CNNSIQRHLE ASCHRHPMLP PDNMWSSQRF QAHLQEVDAP KAWSSVIVPG MKAAVIHALQ TSQDNVQCRK ASFELYGADF VFGEDFQPWL IEINASPTMA PSTAVTARLC AGVQADTLRV VIDRRLDRSC DTGAFELIYK QPAVEVPQYV GIRLLVEGST IKKPVPVGHR RTGVRSSLPH LLTQQGSGES KDSGSPTHRS ASRKNARAES LEHTEKPEPA AVASVSGKGK KAPFHFPSLH SKAWLPSPRV HRPQGRVLRL QHDQLVGSKA LSTTGKALMT LPTAKVLMSF PPHPDLKLAP SMLKPGKVGF ELCCTTWRVV LSGGIGEEGH RQRAAPRPSS APGKGLSSTE PCSKTET
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ttll3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product TTLL3 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    TTLL3 (Tubulin tyrosine Ligase-Like Family, Member 3 (TTLL3))
    Andere Bezeichnung
    Ttll3 (TTLL3 Produkte)
    Synonyme
    HOTTL Protein, 4833441J24Rik Protein, AI450050 Protein, zgc:136840 Protein, tubulin tyrosine ligase like 3 Protein, tubulin tyrosine ligase-like family, member 3 Protein, Ttll3 Protein, TTLL3 Protein, ttll3 Protein
    Hintergrund
    Monoglycylase which modifies alpha- and beta-tubulin, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Involved in the side-chain initiation step of the glycylation reaction by adding a single glycine chain to generate monoglycine side chains. Not involved in elongation step of the polyglycylation reaction. {ECO:0000269|PubMed:19524510}.
    Molekulargewicht
    105.4 kDa Including tag.
    UniProt
    A4Q9E5
Sie sind hier:
Kundenservice