DHX8 Protein (AA 1-1244) (Strep Tag)

Produktnummer ABIN3130674

Produktdetails

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1244

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses DHX8 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAVAVAAAGV LMGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST SKDPVVKPKT EKEKLRELFP VLCQPDNPSA RTMLDEEDVK VAVDVLKELE ALMPSAAGQE KQRDPEHRDR TKKKKRSRSR DRDRDRDRDR DRDRDRDRDR DKDRERDRDR ERDRERDRER DHKRRHRSRS RSHSRTRERT KGKSRYRSRS RSQSPFKDRK DREKYGERNL DRWRDKHVDR PPPEEPAIGD IYNGKVTSIM QFGCFVQLEG LRKRWEGLVH ISELRREGRV ANVADVVSKG QRVKVKVLSF TGTKTSLSMK DVDQETGEDL NPNRRRNLVG ETNEETSMRN PDRPTHLSLV SAPEVEDDSL ERKRLTRISD PEKWEIKQMI AANVLSKEEF PDFDEETGIL PKVDDEEDED LEIELVEEEP PFLRGHTKQS MDMSPIKIVK NPDGSLSQAA MMQSALAKER RELKQAQREA EMDSIPMGLN KHWVDPLPDA EGRQIAANMR GIGMMPNDIP EWKKHAFGGN KASYGKKTQM SILEQRESLP IYKLKEQLVQ AVHDNQILIV IGETGSGKTT QITQYLAEAG YTSRGKIGCT QPRRVAAMSV AKRVSEEFGC CLGQEVGYTI RFEDCTSPET VIKYMTDGML LRECLIDPDL TQYAIIMLDE AHERTIHTDV LFGLLKKTVQ KRQDMKLIVT SATLDAVKFS QYFYEAPIFT IPGRTYPVEI LYTKEPETDY LDASLITVMQ IHLTEPPGDI LVFLTGQEEI DTACEILYER MKSLGPDVPE LIILPVYSAL PSEMQTRIFD PAPPGSRKVV IATNIAETSL TIDGIYYVVD PGFVKQKVYN SKTGIDQLVV TPISQAQAKQ RAGRAGRTGP GKCYRLYTER AYRDEMLTTN VPEIQRTNLA STVLSLKAMG INDLLSFDFM DAPPMETLIT AMEQLYTLGA LDDEGLLTRL GRRMAEFPLE PMLCKMLIMS VHLGCSEEML TIVSMLSVQN VFYRPKDKQA LADQKKAKFH QTEGDHLTLL AVYNSWKNNK FSNPWCYENF IQARSLRRAQ DIRKQMLGIM DRHKLDVVSC GKSTVRVQKA ICSGFFRNAA KKDPQEGYRT LIDQQVVYIH PSSALFNRQP EWVVYHELVL TTKEYMREVT TIDPRWLVEF APAFFKVSDP TKLSKQKKQQ RLEPLYNRYE EPNAWRISRA FRRR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Andere Bezeichnung: Dhx8 (DHX8 Produkte)

Synonyme: LRRGT00035 Protein, DHX8 Protein, im:7153552 Protein, DDX8 Protein, HRH1 Protein, PRP22 Protein, PRPF22 Protein, Ddx8 Protein, mDEAH6 Protein, mKIAA4096 Protein, DEAH-box helicase 8 Protein, DEAH (Asp-Glu-Ala-His) box polypeptide 8 Protein, Dhx8 Protein, DHX8 Protein, dhx8 Protein

Hintergrund: ATP-dependent RNA helicase DHX8 (EC 3.6.4.13) (DEAH box protein 8),FUNCTION: Involved in pre-mRNA splicing as component of the spliceosome. Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. {ECO:0000250|UniProtKB:Q14562}.

Molekulargewicht: 142.6 kDa

UniProt: A2A4P0