F-Box Protein 17 (FBXO17) (AA 1-286) protein (His tag)

Details zu Produkt Nr. ABIN3130617, Anbieter: Anmelden zum Anzeigen
Proteinname
  • FBG4
  • FBX26
  • FBXO26
  • Fbx17
  • FBXO17
  • Fbg4
  • Fbxo26
  • F-box protein 17
  • FBXO17
  • Fbxo17
Proteineigenschaft
AA 1-286
1
1
1
1
Spezies
Maus
3
1
Quelle
Insektenzellen
1
1
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
His tag
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Sequenz MGARPSRRQM TEARRLALTK LPPELLVQVL SHVPPRALVT RCRPVCRAWR DLVDGPSIWL LQLARDRSAE GRALYALAQR CPADADADAD ADGNRHDEFP FCALARFCLR APFGRNLIHN SCGEQGFRGW EVEHGGNGWA VEKNLTLVPG APSQTCFVTS FEWCSKRQLV DLVKEGVWQE LLDSGQIEIC IADWWGAREN CGCIYRLRVR LLDEYENEVV KFSASPNPVL QWTERSCRQV SHVFTNFGKG IRYVSFEQYG RDTRSWVGHY GALVTHSSVR VRIRLS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Fbxo17 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Reinigung Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin free.
Güteklasse Crystallography grade
Proteinname
Hintergrund Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides. Also recognizes sulfated glycans. Does not bind high-mannose glycoproteins (By similarity). {ECO:0000250}.
Molekulargewicht 33.5 kDa Including tag.
UniProt Q9QZM8
Applikations-hinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Kommentare

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for F-Box Protein 17 (FBXO17) (AA 1-286) protein (His tag) (ABIN3130617) „Crystallography Grade“ protein due to multi-step, protein-specific purification process