FBP1 Protein (AA 2-338) (His tag)

Produktnummer ABIN3130443

Produktdetails

Target: FBP1 (Fructose-1,6-Bisphosphatase 1 (FBP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-338

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses FBP1 Protein ist gelabelt mit His tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequenz: ANHAPFETDI STLTRFVMEQ GRKAQGTGEL TQLLNSLCTA IKAISSAVRQ AGIAQLYGIA GSTNVTGDQV KKLDILSNDL VINMLKSSYA TCVLVSEENT NAIIIEPEKR GKYVVCFDPL DGSSNIDCLV SIGTIFGIYR KKSTDEPSEK DALQPGRDLV AAGYALYGSA TMLVLAMDCG VNCFMLDPSI GEFIMVDRDV KMKKKGNIYS LNEGYAKDFD PAINEYLQRK KFPPDGSAPY GARYVGSMVA DIHRTLVYGG IFLYPANKKS PSGKLRLLYE CNPIAYVMEK AGGLATTGDK DILDIVPTEI HQKAPVVMGS SEDVQEFLEI YRKHKAK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Fbp1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: FBP1 (Fructose-1,6-Bisphosphatase 1 (FBP1))

Andere Bezeichnung: Fbp1 (FBP1 Produkte)

Synonyme: FBP Protein, Fbp-2 Protein, Fbp2 Protein, Fbp3 Protein, FBP1 Protein, fbp2 Protein, Fdp Protein, CG10611 Protein, CG31692 Protein, Dmel\\CG31692 Protein, FbPase Protein, fbp1 Protein, cb598 Protein, fb57b01 Protein, zgc:64127 Protein, id:ibd1091 Protein, wu:fb17g10 Protein, wu:fb57b01 Protein, fdp Protein, xcc-b100_0105 Protein, fbp1l Protein, fk92h02 Protein, wu:fk92h02 Protein, zgc:64096 Protein, fbp Protein, fbp1.S Protein, fructose-bisphosphatase 1 Protein, fructose bisphosphatase 1 Protein, Fructose-1,6-bisphosphatase Protein, fructose-1,6-bisphosphatase Protein, fructose-1,6-bisphosphatase 1 Protein, fructose-bisphosphatase class I Protein, fructose-1,6-bisphosphatase 1b Protein, fbp Protein, fructose-1,6-bisphosphatase 1a Protein, fructose-bisphosphatase 1 L homeolog Protein, FBP1 Protein, Fbp1 Protein, fbp1 Protein, LOC100136618 Protein, RR_RS07410 Protein, fbp Protein, fbp1b Protein, fbp1a Protein, fbp1.L Protein

Hintergrund: Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity, increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain. {ECO:0000269|PubMed:16497803, ECO:0000269|PubMed:18375435, ECO:0000269|PubMed:20719858}.

Molekulargewicht: 37.7 kDa Including tag.

UniProt: Q9QXD6

Pathways: Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Dicarboxylic Acid Transport