CK1 epsilon Protein (AA 1-416) (Strep Tag)

Produktnummer ABIN3130397

Produktdetails

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-416

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses CK1 epsilon Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Sequenz: MELRVGNKYR LGRKIGSGSF GDIYLGANIA SGEEVAIKLE CVKTKHPQLH IESKFYKMMQ GGVGIPSIKW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFST YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGAARN PEDVDRERRE HEREERMGQL RGSATRALPP GPPTGATANR LRSAAEPVAS TPASRIQQTG NTSPRAISRA DRERKVSMRL HRGAPANVSS SDLTGRQEVS RLAASQTSVP FDHLGK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Andere Bezeichnung: Csnk1e (CSNK1E Produkte)

Synonyme: CKIepsilon Protein, HCKIE Protein, CKIe Protein, ck1-epsilon Protein, ck1epsilon Protein, hckie Protein, dco Protein, fj38c01 Protein, wu:fj38c01 Protein, wu:fj62a04 Protein, zgc:77310 Protein, AI426939 Protein, AI551861 Protein, AW457082 Protein, CK1epsilon Protein, KC1epsilon Protein, tau Protein, ckie Protein, CSNK1E Protein, Csnk1e Protein, CKI-epsilon Protein, 0538/13 Protein, 0915/10 Protein, 1396/02 Protein, 1447/01 Protein, 1460/09 Protein, CG2048 Protein, CK1delta/epsilon Protein, DBT Protein, DBT/CK1epsilon Protein, DCO Protein, DCO/CKIe Protein, Dbt Protein, Dco Protein, Dco/CK1 Protein, Dmel\CG2048 Protein, dCKIepsilon Protein, dbt Protein, dco-1 Protein, ddbt Protein, l(3)S053813 Protein, l(3)S144701 Protein, l(3)dco Protein, l(3)dco-1 Protein, l(3)discs overgrown Protein, l(3)discs overgrown-1 Protein, l(3)j3B9 Protein, l(3)rK215 Protein, casein kinase 1 epsilon Protein, casein kinase 1, epsilon Protein, casein kinase 1 epsilon L homeolog Protein, casein kinase I isoform epsilon Protein, casein kinase I epsilon Protein, casein kinase I Protein, LOC400927-CSNK1E readthrough Protein, discs overgrown Protein, CSNK1E Protein, csnk1e Protein, Csnk1e Protein, csnk1e.L Protein, LOC443240 Protein, NAEGRDRAFT_82890 Protein, LOC100407384 Protein, LOC100523244 Protein, LOC100607246 Protein, LOC100727500 Protein, LOC400927-CSNK1E Protein, dco Protein

Hintergrund: Casein kinase I isoform epsilon (CKI-epsilon) (CKIe) (EC 2.7.11.1),FUNCTION: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (By similarity). Participates in Wnt signaling (By similarity). Phosphorylates DVL1 (By similarity). Phosphorylates DVL2 (By similarity). Phosphorylates NEDD9/HEF1 (PubMed:29191835). Central component of the circadian clock (PubMed:18400165, PubMed:19414593, PubMed:21930935). In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation (PubMed:18400165, PubMed:19414593, PubMed:21930935). Controls PER1 and PER2 nuclear transport and degradation (PubMed:10848614, PubMed:14701732, PubMed:18400165, PubMed:19414593, PubMed:21930935). Inhibits cytokine-induced granuloytic differentiation (By similarity). {ECO:0000250|UniProtKB:P49674, ECO:0000269|PubMed:10848614, ECO:0000269|PubMed:14701732, ECO:0000269|PubMed:18400165, ECO:0000269|PubMed:19414593, ECO:0000269|PubMed:21930935, ECO:0000269|PubMed:29191835}.

Molekulargewicht: 47.3 kDa

UniProt: Q9JMK2

Pathways: Hedgehog Signalweg, M Phase