UTP3 Protein (AA 1-469) (Strep Tag)

Produktnummer ABIN3130025

Produktdetails

Target: UTP3 (UTP3, Small Subunit (SSU) Processome Component (UTP3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-469

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses UTP3 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MVKKSRRRGA AQWAAVRAQA GLTATDENED DLGLPPSPGD SSYYQDQVDE FHEARSRAVL AKGWNEVESG EEDGDEEEEV LPLDIDDGDD EDGESSEEEE VGEDDDGGSS VQSEAEASVD PSLSWGQRKK LYYDTDYGSK SRGRQSQQEV EEEEREEEEE AQIIQRRLAQ ALQEDDFGVA WVEAFAKPVP QVDEAETRVV KDLAKVSVKE KLKMLKKESP ELLELIEDLQ AKLTEVKDEL EPLLQLVEKG VIPTGRGSEY LKTKYNLYLN YCANISFYLI LKARRVPAHG HPVIERLVTY RNLINKLSVV DQKLSSEIRH LLTAKDGAVK KEMTPKAKLT KTKPKSVKQA AAVALTDEPD FDGAALKYYK EMEDRQELKR KKEENSAEEQ ALEEQNAKRA ITYQIAKNRG LTPRRKKIDR NPRVKHREKF RKAKIRRRGQ VREVRREEQR YSGELSGIRA GVKKSIKLK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: UTP3 (UTP3, Small Subunit (SSU) Processome Component (UTP3))

Andere Bezeichnung: Utp3 (UTP3 Produkte)

Synonyme: CG4202 Protein, Dmel\\CG4202 Protein, dSAS10 Protein, l(1)G0106 Protein, sas-10 Protein, sas10 Protein, CRL1 Protein, CRLZ1 Protein, SAS10 Protein, 2400011K09Rik Protein, AW557551 Protein, C87704 Protein, Crl1 Protein, Crlz1 Protein, Sas10 Protein, Something about silencing 10 Protein, UTP3, small subunit processome component Protein, UTP3 small subunit processome component Protein, Sas10 Protein, UTP3 Protein, Utp3 Protein

Hintergrund: Something about silencing protein 10 (Charged amino acid-rich leucine zipper 1) (Crl-1) (Disrupter of silencing SAS10) (UTP3 homolog),FUNCTION: Essential for gene silencing: has a role in the structure of silenced chromatin. Plays a role in the developing brain. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (By similarity). {ECO:0000250|UniProtKB:Q12136, ECO:0000250|UniProtKB:Q9NQZ2, ECO:0000269|PubMed:11404095}.

Molekulargewicht: 53.4 kDa

UniProt: Q9JI13