RAI14 Protein (AA 1-979) (Strep Tag)

Produktnummer ABIN3129724

Produktdetails

Target: RAI14 (Retinoic Acid Induced 14 (RAI14))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-979

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses RAI14 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MKSLKAKFRK SDTNEWNKND DRLLQAVENG DAEKVASLLG KKGASATKHD SEGKTAFHLA AAKGHVECLK VMVTHGVDVT AQDSSGHSAL HVAAKNGHPE CIRKLLQYKS PAENIDNSGK TALHYAAAQG CLQAVQLLCE HKSPINLKDL DGNIPLLVAV QNGHSEACHF LLDHGADVNS RDKNGRTALM LACETGSSNT VDALIKKGAD LSLVDSLGHN ALHYSKLSEN AGIQNLLLSK ISQDADLKTP TKPKQHDQVS KISSERSGTP KKRKAPPPPI SPTQLSDVSS PRSITSTPLS GKESVFFAEA PFKAEISSIQ ENKDRLSDST AGADSLLDIS SEADQQDLLV LLQAKVASLT LHNKELQDKL QAKSPKDKEA EADLSFQSFH STQTDLAPSP GKASDIPSSD AKSSPPVEHP AGTSTTDNDV IIRQLQDSLH DLQKRLESSE AEKKQLQDEL QSQRTDTLCL NNTEISENGS DLSQKLKETQ SKYEEAMKEV LSVQKQMKLG LLSQESADGY SHLREAPADE DIDTLKQDLQ KAVEESARNK ERVRELETKL AEKEQAEATK PPAEACEELR SSYCSVIENM NKEKAFLFEK YQQAQEEIMK LKDTLKSQMP QEAPDDSGDM KEAMNRMIDE LNKQVSELSQ LYREAQAELE DYRKRKSLED AAEYIHKAEH ERLMHVSNLS RAKSEEALSE MKSQYSKVLN ELTQLKQLVD AHKENSVSIT EHLQVITTLR TTAKEMEEKI SALTGHLANK EAEVAKLEKQ LAEEKAAVSD AMVPKSSYEK LQASLESEVN ALATKLKESV REREKAHSEV AQVRSEVSQA RREKDNIQTL LKAKEQEVTA LVQKFQRAQE ELAGMRRCSE TSSKLEEDKD EKINEMTREV LKLKEALNSL SQLSYSTSSS KRQSQQLDLL QQQVKQLQNQ LAECKKHHQE VISVYRMHLL YAVQGQMDED VQKVLKQILT MCKNQSQKK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RAI14 (Retinoic Acid Induced 14 (RAI14))

Andere Bezeichnung: Rai14 (RAI14 Produkte)

Synonyme: RAI14 Protein, NORPEG Protein, RAI13 Protein, 1700008J19Rik Protein, 1700020L11Rik Protein, Ankycorbin Protein, Norpeg Protein, mKIAA1334 Protein, retinoic acid induced 14 Protein, ankycorbin Protein, RAI14 Protein, rai14 Protein, LOC100541535 Protein, Rai14 Protein

Hintergrund: Ankycorbin (Ankyrin repeat and coiled-coil structure-containing protein) (Novel retinal pigment epithelial cell protein) (Retinoic acid-induced protein 14) (p125),FUNCTION: Plays a role in actin regulation at the ectoplasmic specialization, a type of cell junction specific to testis. Important for establishment of sperm polarity and normal spermatid adhesion. May also promote integrity of Sertoli cell tight junctions at the blood-testis barrier. {ECO:0000250|UniProtKB:Q5U312}.

Molekulargewicht: 108.9 kDa

UniProt: Q9EP71