NUDT21 Protein (AA 2-227) (His tag)

Produktnummer ABIN3128302

Produktdetails

Target: NUDT21 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 21 (NUDT21))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-227

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses NUDT21 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: SVVPPNRSQT GWPRGVNQFG NKYIQQTKPL TLERTINLYP LTNYTFGTKE PLYEKDSSVA ARFQRMREEF DKIGMRRTVE GVLIVHEHRL PHVLLLQLGT TFFKLPGGEL NPGEDEVEGL KRLMTEILGR QDGVLQDWVI DDCIGNWWRP NFEPPQYPYI PAHITKPKEH KKLFLVQLQE KALFAVPKNY KLVAAPLFEL YDNAPGYGPI ISSLPQLLSR FNFIYN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Nudt21 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NUDT21 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 21 (NUDT21))

Andere Bezeichnung: Nudt21 (NUDT21 Produkte)

Synonyme: CFIM25 Protein, CPSF5 Protein, 25kDa Protein, 3110048P04Rik Protein, 5730530J16Rik Protein, AU014860 Protein, AW549947 Protein, Cpsf5 Protein, zgc:63966 Protein, cpsf5 Protein, An16g01870 Protein, AO090003001316 Protein, Afu5g02030 Protein, T9L24.30 Protein, T9L24_30 Protein, atnudt21 Protein, nudix hydrolase homolog 21 Protein, nudix hydrolase 21 Protein, nudix (nucleoside diphosphate linked moiety X)-type motif 21 Protein, nudix hydrolase 21 L homeolog Protein, cleavage and polyadenylation specificity factor subunit 5 Protein, cleavage and polyadenylation specific factor 5 Protein, autocrine motility factor receptor Protein, nudix hydrolase homolog 21 Protein, NUDT21 Protein, Nudt21 Protein, nudt21 Protein, nudt21.L Protein, ANI_1_1518144 Protein, AOR_1_2312154 Protein, PTRG_11035 Protein, PAAG_04662 Protein, MCYG_02022 Protein, VDBG_06706 Protein, MGYG_04057 Protein, cpsf5 Protein, PGTG_05353 Protein, Tsp_03672 Protein, LOC732888 Protein, AFUA_5G02030 Protein, NFIA_040090 Protein, ACLA_003240 Protein, CC1G_07993 Protein, PMAA_039710 Protein, AFLA_025180 Protein, TSTA_079060 Protein, BDBG_01565 Protein, TERG_04393 Protein

Hintergrund: Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export (By similarity). {ECO:0000250}.

Molekulargewicht: 27.1 kDa Including tag.

UniProt: Q9CQF3