DDO Protein (AA 1-341) (Strep Tag)

Produktnummer ABIN3127737

Produktdetails

Target: DDO (D-Aspartate Oxidase (DDO))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-341

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses DDO Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MDTVCIAVVG AGVIGLSTAA CISQLVPGCT VTVISDRFTP DTTSNVAAGM LIPHTCADTP VPTQKRWFRE TFEHLSEIAK SAEAADAGVH LVSGWQIFRS VPAEEVPFWA DVVLGFRKMT EAELKRFPQY VFGQAFTTLK CETSAYLPWL ERRIKGSGGL LLTRRIEDLW ELQPSFDIVV NCSGLGSRRL VGDPMISPVR GQVLQARAPW VKHFIRDGGG LTYVYPGMSY VTLGGTRQKG DWNRSPDAEL SREIFSRCCT LEPSLHRAYD IKEKVGLRPS RPGVRLQKEI LVRGQQTLPV VHNYGHGSGG ISVHWGSALE ATRLVMECIH TLRTPASLSK L
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DDO (D-Aspartate Oxidase (DDO))

Andere Bezeichnung: Ddo (DDO Produkte)

Synonyme: DDO Protein, DDBDRAFT_0203041 Protein, DDBDRAFT_0238431 Protein, DDB_0203041 Protein, DDB_0238431 Protein, DDBDRAFT_0217305 Protein, DDBDRAFT_0238432 Protein, DDB_0217305 Protein, DDB_0238432 Protein, 5330420D20Rik Protein, 5730402C02Rik Protein, AI467244 Protein, DASOX Protein, DDO-1 Protein, DDO-2 Protein, D-aspartate oxidase Protein, D-amino-acid:oxygen oxidoreductase Protein, DDO Protein, IFG3 Protein, LACBIDRAFT_308911 Protein, PAS_chr3_0406 Protein, Amir_4180 Protein, Gbro_4657 Protein, ddo-2 Protein, ddo-1 Protein, Sros_8818 Protein, Kfla_2239 Protein, Gobs_3505 Protein, Snas_1168 Protein, ddo Protein, Ddo Protein

Hintergrund: D-aspartate oxidase (DASOX) (DASPO) (DDO) (EC 1.4.3.1),FUNCTION: Selectively catalyzes the oxidative deamination of acidic amino acids (PubMed:17469229, PubMed:18235994, PubMed:20603179, PubMed:20567862, PubMed:25747990, PubMed:26058797, PubMed:26961959, PubMed:28393897, PubMed:29292239, PubMed:32185508, PubMed:32553892, PubMed:37189369). Suppresses the level of D-aspartate in the brain, an amino acid that can act as an agonist for glutamate receptors (PubMed:26961959, PubMed:32185508, PubMed:35915065). Protects the organism from the toxicity of D-amino acids (By similarity). May also function in the intestine (PubMed:35915065). {ECO:0000250|UniProtKB:D3ZDM7, ECO:0000269|PubMed:17469229, ECO:0000269|PubMed:18235994, ECO:0000269|PubMed:20567862, ECO:0000269|PubMed:20603179, ECO:0000269|PubMed:25747990, ECO:0000269|PubMed:26058797, ECO:0000269|PubMed:26961959, ECO:0000269|PubMed:28393897, ECO:0000269|PubMed:29292239, ECO:0000269|PubMed:32185508, ECO:0000269|PubMed:32553892, ECO:0000269|PubMed:35915065, ECO:0000269|PubMed:37189369}., FUNCTION: [Isoform 1]: Selectively catalyzes the oxidative deamination of acidic amino acids. {ECO:0000269|PubMed:16516413}., FUNCTION: [Isoform 2]: Does not exhibit D-aspartate oxidase activity. {ECO:0000269|PubMed:16516413}.

Molekulargewicht: 37.5 kDa

UniProt: Q922Z0