SMEK2 Protein (AA 1-820) (His tag)
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- Target Alle SMEK2 Produkte
- SMEK2 (SMEK Homolog 2, Suppressor of Mek1 (Dictyostelium) (SMEK2))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-820
- Spezies
- Maus
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses SMEK2 Protein ist gelabelt mit His tag.
- Applikation
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequenz
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MSDTRRRVKV YTLNEDRQWD DRGTGHVSST YVEELKGMSL LVRAESDGSL LLESKINPNT AYQKQQDTLI VWSEAENYDL ALSFQEKAGC DEIWEKICQV QGKDPSVEVT QDLIDESEEE RFEEMPETSH LIDLPACELS KLEEIADLVT SVLSSPIRRE KLALALENEG YIKKLLQLFQ ACENLENTEG LHHLYEIIRG ILFLNKATLF EVMFSDECIM DVVGCLEYDP ALAQPKRHRE FLTKTAKFKE VIPITDSELR QKIHQTYRVQ YIQDIILPTP SVFEENFLST LTSFIFFNKV EIVSMLQEDE KFLSEVFAQL TDEATDDDKR RELVNFFKEF CAFSQTLQPQ NRDAFFKTLA KLGILPALEI VMGMDDLQVR SAATDIFSYL VEFSPSMVRE FVMQEAQQSD DDVLLINVVI EQMICDTDPE LGGAVQLMGL LRTLIDPENM LATTNKTEKS EFLNFFYNHC MHVLTAPLLT NTSEDKCEKD NMLGSNTTNT ICPDNYQTAQ LLALILELLT FCVEHHTYHI KNYIMNKDLL RRVLVLMNSK HTFLALCALR FMRRIIGLKD EFYNRYITKG NLFEPVINAL LDNGTRYNLL NSAVIELFEF IRVEDIKSLT AHIVENFYKA LESIEYVQTF KGLKTKYEQE KDRQNQKLNS VPSILRSNRF RRDAKALEED EEMWFNEDDD EEGKAVITPV EKSKTEDDFP DSYEKFMETK KAKESEDKEN LPKRASSGGF KFTFSHSPSA TNGTNSTNSK SVVSQTTPAS SNVASSKTTS LATSVTATKG NLVGLVDYPD DEEEDEEEES SPRKRPRLGS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ppp4r3b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- SMEK2 (SMEK Homolog 2, Suppressor of Mek1 (Dictyostelium) (SMEK2))
- Andere Bezeichnung
- Ppp4r3b (SMEK2 Produkte)
- Synonyme
- FLFL2 Protein, PP4R3B Protein, PSY2 Protein, smk1 Protein, AW011752 Protein, AW557776 Protein, mKIAA1387 Protein, flfl Protein, zgc:56492 Protein, zgc:77352 Protein, wu:fj55c11 Protein, RGD1309450 Protein, flfl2 Protein, pp4r3b Protein, psy2 Protein, smek2-b Protein, MGC79482 Protein, Smek3p Protein, Smek2 Protein, SMEK2 Protein, protein phosphatase 4 regulatory subunit 3B Protein, protein phosphatase 4, regulatory subunit 3B Protein, protein phosphatase 4 regulatory subunit 3B S homeolog Protein, RIKEN cDNA 4930415L06 gene Protein, protein phosphatase 4 regulatory subunit 3B L homeolog Protein, PPP4R3B Protein, Ppp4r3b Protein, ppp4r3b Protein, ppp4r3b.S Protein, 4930415L06Rik Protein, ppp4r3b.L Protein
- Hintergrund
- Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers (By similarity). {ECO:0000250}.
- Molekulargewicht
- 94.9 kDa Including tag.
- UniProt
- Q922R5
- Pathways
- Regulation of Carbohydrate Metabolic Process
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