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DDX27 Protein (AA 1-760) (His tag)

Crystallography grade DDX27 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, Crys, ELISA, WB
Produktnummer ABIN3127666
  • Target Alle DDX27 Proteine anzeigen
    DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-760
    Spezies
    Maus
    Quelle
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses DDX27 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Crystallization (Crys), ELISA, Western Blotting (WB)
    Sequenz
    MLAELGFIRT IGENDEVPVE PESDSGDEEE EGPIVLGRKQ KALQKNRSAD FNPDFVFTEK EGMYDGSWAL ADVMSQLKKK RAATTLDEKI EKVRKRRKAE DKEAKSGKVE EKEGQADSDL KGQENPGEDE AGSKDEDSET DYSSEDEEIL TKADTLKVKE KKKKKKGQAA GGFFEDASEY DKSLSFQDMN LSRPLLKAIT AMGFKQPTPI QKACIPVGLL GKDICACAAT GTGKTAAFAL PVLERLIYKP RQAAVTRVLV LVPTRELGIQ VHSVTKQLAQ FCSITTCLAV GGLDVKSQEA ALRAAPDILI ATPGRLIDHL HNCPSFHLSS IEVLILDEAD RMLDEYFEEQ MKEIIRMCSH HRQTMLFSAT MTDEVKDLAS VSLKNPVRIF VNSNTDVAPF LRQEFIRIRP NREGDREAIV AALLMRTFTD HVMLFTQTKK QAHRMHILLG LLGLQVGELH GNLSQTQRLE ALRRFKDEQI DILVATDVAA RGLDIEGVKT VINFTMPNTV KHYVHRVGRT ARAGRAGRSV SLVGEEERKM LKEIVKAAKA PVKARILPQD VILKFRDKIE KLEKDVYAVL QLEAEEKEMQ QSEAQIDTAQ RLLAKGKETA DQEPERSWFQ TKEERKKEKI AKALQEFDLA LRGKKKRKKF MKDAKKKGEM TAEERSQFEI LKAQMFAERL AKRNRRTKRA RAMPEDEPTG PAKKQKQQQK SVFDEELTNT SKKALKQYRA GPSFEERKQS GLPRQRRGNF KSKSRYKRKK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ddx27 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))
    Andere Bezeichnung
    Ddx27 (DDX27 Produkte)
    Synonyme
    cb843 Protein, DDX27 Protein, MGC114699 Protein, rhlp Protein, rrp3p Protein, pp3241 Protein, hspc259 Protein, DRS1 Protein, Drs1p Protein, PP3241 Protein, RHLP Protein, dJ686N3.1 Protein, C86129 Protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 Protein, DEAD-box helicase 27 Protein, DEAD-box helicase 27 L homeolog Protein, ddx27 Protein, DDX27 Protein, ddx27.L Protein, Ddx27 Protein
    Hintergrund
    Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA. {ECO:0000250|UniProtKB:Q96GQ7}.
    Molekulargewicht
    86.9 kDa Including tag.
    UniProt
    Q921N6
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