Olfactory Receptor 56 (OLFR56) (AA 1-315) protein (rho-1D4 tag)

Details zu Produkt Nr. ABIN3127139, Anbieter: Anmelden zum Anzeigen
Proteinname
  • IF7
  • MOR276-1
  • BmOr-56
  • olfactory receptor 56
  • Olfr56
  • Olr56
  • Or-56
Proteineigenschaft
AA 1-315
Spezies
Maus
Quelle
Insektenzellen
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
rho-1D4 tag
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Sequenz MGIWLNESSV DGFILLGIFS QSQTDLLLFS TVMLVFTVAL CGNVLLILLI YTDPRLHTPM YFFLSQLSLM DLMLVCNIVP KMAVNFLSGR KSISFAGCGI QIGFFVSLVG SEGLLLGLMA YDRYVAISHP LHYPILMSQK VCLQIAGSSW AFGILDGIIQ MVAAMSLPYC GSRYIDHFFC EVPALLKLAC ADTSLFDTLL FACCVFMLLL PFSIIVTSYA RILGAVLRMH SAQSRKKALA TCSSHLTAVS LFYGAAMFIY LRPRRYRAPS HDKVVSIFYT VLTPMLNPLI YSLRNREVMG ALRKGLDRCR VGSQH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Olfr56 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Reinigung Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin-free.
Güteklasse Crystallography grade
Hintergrund Odorant receptor. Activated by (+) and (-)-limonene. {ECO:0000269|PubMed:9875846}.
Molekulargewicht 36.2 kDa Including tag.
UniProt Q8VGD6
Forschungsgebiet Neurology
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Kommentare

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Olfactory Receptor 56 (OLFR56) (AA 1-315) protein (rho-1D4 tag) (ABIN3127139) „Crystallography Grade“ protein due to multi-step, protein-specific purification process