TAF9 Protein (AA 1-172) (Strep Tag)

Produktnummer ABIN3126947

Produktdetails

Target: TAF9 (RNA Polymerase II TBP-Associated Factor Subunit G (TAF9))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-172

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TAF9 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MKLPNILLTG TPGVGKTTLG KELASRSGLK YVNVGDLARE GQLYDGYDEE YGCPILDEDR VVDELEHQMQ EGGVIVDYHG CDFFPERWFH IVFVLRTDNG VLYKRLETRG YNEKKLQDNI QCEIFQVLYE EAIASYKEEI VHQLPSNEPE QLEDNINQIS KWIEQWVKDH NP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TAF9 (RNA Polymerase II TBP-Associated Factor Subunit G (TAF9))

Andere Bezeichnung: Ak6 (TAF9 Produkte)

Synonyme: AK6 Protein, CGI-137 Protein, CINAP Protein, CIP Protein, MGC:1603 Protein, MGC:3647 Protein, MGC:5067 Protein, TAF2G Protein, TAFII31 Protein, TAFII32 Protein, TAFIID32 Protein, hCINAP Protein, 2310012M09Rik Protein, 2810046E22Rik Protein, 4921516M08Rik Protein, AA673500 Protein, AU045423 Protein, Ak6 Protein, Taf2g Protein, im:7089279 Protein, wu:fb16h03 Protein, zgc:153410 Protein, TATA-box binding protein associated factor 9 Protein, adenylate kinase 6 L homeolog Protein, adenylate kinase isoenzyme 6-like Protein, SAGA complex/ transcription initiation factor Taf9 Protein, TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor Protein, TAF9 Protein, Taf9 Protein, ak6.L Protein, LOC100355082 Protein, taf9 Protein

Hintergrund: Adenylate kinase isoenzyme 6 (AK6) (EC 2.7.4.3) (Coilin-interacting nuclear ATPase protein) (Dual activity adenylate kinase/ATPase) (AK/ATPase),FUNCTION: Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis. May be involved in regulation of Cajal body (CB) formation. {ECO:0000255|HAMAP-Rule:MF_03173}.

Molekulargewicht: 19.9 kDa

UniProt: Q8VCP8

Pathways: Negative Regulation of intrinsic apoptotic Signaling