GGA1 Protein (AA 1-635) (Strep Tag)

Produktnummer ABIN3126561

Produktdetails

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-635

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses GGA1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MEPAMEPETL EARINRATNP LNKELNWASI NSFCEQLNED FEGPPLATRL LAHKIQSPQE WEAIQALTVL ETCMKSCGKR FHDEVGKFRF LNELIKVVSP KYLGSRTSEK VKSKILELLY SWTVCLPEEV KIAEAYQMLK KQGIVKSDPK LPEDAIFPLP PPRPKNVIFE DEEKSKMLAR LLKSSHPEDL RAANKLIKEM VQEDQKRMEK ISKRVNAIEE VNNNVKLLTE MVMSHSQGAA SSSSEDLMKE LYQRCERMRP TLFRLASDTE DNDEALAEIL QANDNLTQVI NLYKQLVRGE EVNGDATASS IPGSTSALLD LSGLDLPPPG TTQPATPTRP GNQSSPEQLS ASVSLLDDEL MSLGLSDPTP PSGTSSDSVG WDNFQSSDGT ESSVPPPAQA PSMDCRPPAQ APPPTSSGLD DLDLLGKTLM QQALPPEAQQ VRWEKQQPAP RLTLRDLQSK SSSPSPGAAS LLHTTSPEPP GPPPQATPTE FSLTSITVPL ESIKPSSILP VTVYDQHGFR VLFHFARDPL PGRSDVLVVV VSMLSTAPQP IRNIVFQSAV PKVMKVRLQP PSGTELPAFN PIVHPSAITQ VLLLANPQKE KVRLRYKLIF TMGDQTYNEM GDVDQFPPPE TWGSL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Andere Bezeichnung: Gga1 (GGA1 Produkte)

Synonyme: 4930406E12Rik Protein, AU016030 Protein, AW209092 Protein, fb33h10 Protein, im:6912200 Protein, wu:fb33h10 Protein, zgc:101044 Protein, putative golgi associated, gamma adaptin ear containing,arf binding protein 1 (gga1) Protein, golgi associated, gamma adaptin ear containing, ARF binding protein 1 Protein, golgi-associated, gamma adaptin ear containing, ARF binding protein 1 Protein, Smp_164030 Protein, Gga1 Protein, gga1 Protein, GGA1 Protein

Hintergrund: ADP-ribosylation factor-binding protein GGA1 (Gamma-adaptin-related protein 1) (Golgi-localized, gamma ear-containing, ARF-binding protein 1),FUNCTION: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes (PubMed:25405894). Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface (By similarity). Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (PubMed:25405894). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (By similarity). {ECO:0000250|UniProtKB:Q9UJY5, ECO:0000269|PubMed:25405894}.

Molekulargewicht: 70.0 kDa

UniProt: Q8R0H9