TRIM68 Protein (AA 1-485) (Strep Tag)

Produktnummer ABIN3126306

Produktdetails

Target: TRIM68 (Tripartite Motif Containing 68 (TRIM68))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-485

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TRIM68 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MDPAVLMEAI VEEVNCPICM TFLREPVSIS CGHTFCHSCL SGLWKLPGES QNLSYTCPLC RAPVKPRKLR PNWQLASVVD KVRLLGFCME MGLKTDVCDL HKEQLTMFCK EDDMVTCEAC KQSPEHEAHS VVPIKDVAWE YKWKLQQALE HLRKEQEEAW KLEVSEKEQA AIWKTQMERR KQSIRWEFEK YRQLLKEKEL PCQQAEEEAA AAQASLEQEK GETASKLELR REAIIRQSQV LWSMIVELEE RSQRPVRWML QGIQEALNRS ESWTLQQLEP ISLELKTDCR VLGLRETLKT FAVDVRLDPD TAYSRLVVSK DRKSVHYGVT QQNLPDNPER FYRYNIVLGS QCISSGRHYW EVEVGDRSEW GLGVCVENVD RKEVVYLSPR YGFWVIRLRK GTEYRAGTDE YPLLPLTVPP HRVGIFLDYE AHDISFYNVT DGASHIFTFP CYPFPGRLLP YFSPCYSIDT NNTTPLTICT LGGEG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TRIM68 (Tripartite Motif Containing 68 (TRIM68))

Andere Bezeichnung: Trim68 (TRIM68 Produkte)

Synonyme: GC109 Protein, RNF137 Protein, SS-56 Protein, SS56 Protein, AI894158 Protein, F730114J12Rik Protein, Rnf137 Protein, TRIM68 Protein, tripartite motif containing 68 Protein, tripartite motif-containing 68 Protein, E3 ubiquitin-protein ligase TRIM68 Protein, TRIM68 Protein, Trim68 Protein, LOC100053519 Protein

Hintergrund: E3 ubiquitin-protein ligase TRIM68 (EC 2.3.2.27) (RING finger protein 137) (RING-type E3 ubiquitin transferase TRIM68) (Tripartite motif-containing protein 68),FUNCTION: Functions as a ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity. {ECO:0000250|UniProtKB:Q6AZZ1}.

Molekulargewicht: 56.1 kDa

UniProt: Q8K243

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling