OSBPL11 Protein (AA 1-751) (Strep Tag)

Produktnummer ABIN3126078

Produktdetails

Target: OSBPL11 (Oxysterol Binding Protein-Like 11 (OSBPL11))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-751

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses OSBPL11 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MQGGEPASVM KVSESEGKLE GLATAVTPNK NSGNSSCGGA ISSSSSNSSR GGSAKGWQYS DHMESVNGYL MKYTNLVTGW QYRFFVLNNE AGLLEYFVNE QSRNQKPRGT LQLAGAVISP SDEDSHTFTV NAASGEQYKL RATDAKERQH WVSRLQICTQ HHTEAIGKNN PPLKSRSFSL ASSGNSPISQ RRPSQNAMSF FNVGHSKLQS VNKRAHLHPD HLVEVREMMS HAEGQQRDLI RRIECLPASG LLSSLDQDLL MLKATSMATM NCLNDCFHIL QLQHASHQKG ALPSGTTIEW LEPKIPLSNH YKNGAEQPFA TEPNKPMGAP EAQCVAESGV LAREPEDISA DDEVEDTCDN KEDDLGAVEE QRSVILHLLS QLKLGMDLTR VVLPTFILEK RSLLEMYADF MSHPDLFIGI TNGATPEDRM IRFVEYYLTS FHEGRKGAIA KKPYNPIIGE TFHCSWRMPK SEVASGVSSS SSTPAITDHA PLPEEAPTQS VSDCYTVRFV AEQVSHHPPV SGFYAECAER KMCVNAHVWT KSKFLGMSIG VTMVGEGVLC LLEHGEEYTF SLPCAYARSI LTVPWVELGG KVSVNCAKTG YSASITFHTK PFYGGKLHRV TAEVKYNLTN TVVCRVQGEW NSVLEFTYSN GETKFVDLAK LAVTKKRVRP LEKQDPFESR RLWKNVTDSL RESEIDKATE HKRSLEERQR TEERLRTETG TPWKTKYFIK EGDGWVYHKP LWKGIPSQPA E
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: OSBPL11 (Oxysterol Binding Protein-Like 11 (OSBPL11))

Andere Bezeichnung: Osbpl11 (OSBPL11 Produkte)

Synonyme: si:dkey-101k6.4 Protein, osbpl11 Protein, MGC107773 Protein, MGC131376 Protein, OSBPL11 Protein, ORP-11 Protein, ORP11 Protein, OSBP12 Protein, TCCCIA00292 Protein, 9430097N02Rik Protein, AI132306 Protein, AU019795 Protein, oxysterol binding protein like 11 Protein, oxysterol-binding protein-related protein 11 Protein, oxysterol binding protein-like 11 Protein, oxysterol binding protein like 11 L homeolog Protein, OSBPL11 Protein, Tsp_10686 Protein, osbpl11 Protein, osbpl11.L Protein, Osbpl11 Protein

Hintergrund: Oxysterol-binding protein-related protein 11 (ORP-11) (OSBP-related protein 11),FUNCTION: Plays a role in regulating ADIPOQ and FABP4 levels in differentiating adipocytes and is also involved in regulation of adipocyte triglyceride storage. Weakly binds 25-hydroxycholesterol. {ECO:0000250|UniProtKB:Q9BXB4}.

Molekulargewicht: 83.6 kDa

UniProt: Q8CI95