SKA3 Protein (AA 1-411) (Strep Tag)

Produktnummer ABIN3125635

Produktdetails

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-411

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SKA3 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MNPIQSFHCK LRGLATTLDS ETARLLRALD GEDSDFEDSP GRILHDLHSE VQTLKDNVNA LLDEARLENQ ESTRFKKATK ILMEKNSADV RKLREFFQKY GYQARDKEDS GCEHRVNNST PELAVCKDIQ KAGVKELSDP CVPSGSVSEE PLRSPQLSDF GLQRYIISQV PANPPQTAAS LKEERVAETP PAKDPSVQVL KTPRCALRMD DFECETPKLE HFGISEHTMC LNEDYTMGLK NMKNIKSSLL SGVSGEAIGT GPVTSDNSFA IPGPIIQQME ENDVEYVSSP LPPKFCTPGL KIPSTMDRTD LVSIDYPLSK PNSSSTDLEI KDCVPLILNS DECYQSFAEP PSSAITSCEN FATPSPPKVT AIPEDILQMI TKHSSNLASP LDVKVMPRRK GTRGAANKEN W
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Andere Bezeichnung: Ska3 (SKA3 Produkte)

Synonyme: C13orf3 Protein, RAMA1 Protein, RGD1307201 Protein, C12H13ORF3 Protein, fc23d11 Protein, si:rp71-68n21.13 Protein, wu:fc23d11 Protein, rama1 Protein, F630043A04Rik Protein, spindle and kinetochore associated complex subunit 3 Protein, spindle and kinetochore associated complex subunit 3 L homeolog Protein, SKA3 Protein, Ska3 Protein, ska3 Protein, ska3.L Protein

Hintergrund: Spindle and kinetochore-associated protein 3,FUNCTION: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. {ECO:0000250|UniProtKB:Q8IX90}.

Molekulargewicht: 45.4 kDa

UniProt: Q8C263