GFR Protein (AA 1-814) (Strep Tag)

Produktnummer ABIN3125572

Produktdetails

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-814

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses GFR Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MTTGDCQTLS RRISNPYLEH SPSQIYGENS SCAGRALRNI IILQAADLVK DRVNLKGFYR RSCVGSELVD WLLEHCPFVQ CRSMAIGVWQ LLLDMGIMSS VDQHLYFQDN YVFYQFSSDE CSYLYCEFER EEEWQKGVKL LLELVHLIPA RAGICDLSHQ KTEDSEESSD EILARLTSAV QRELAAVIAL KARKSAIEQD DENADKHVTV TEANNGPDPQ AGVMCKLQER DDIGRIELVH KLARENCQFL QTEKKESEKL EQQDDEVTMV QVKEQGQSVL VLKKVASCGP APTSGSAEND ARYVVVSGTP EKILEHLLND LHLAEVQHKE TETLLDDFLL TYTVFMTTDD LCQALLRHYS AKKYQGEEEN SDVPCRKRKV LHLVSQWISL YKDWLHEDEH SKMFLKTIYR NVLDDVYEYP ILEKELKEFQ KILGVYRRHT VDEYSPQKKN KALFHQFSLK ENWLQHRGTV AETEEIFCHV YITEHSYISV KAKVSSTAQE ILKVVAEKLQ RAEEDLALVA ITFSGEKHEF QPNDLAISKS LEASGRIYVY RKDLADTLNP LAENEESQQR SMRILGMNTW DLALELMSFD WSLFNSIHEQ ELIYFTFSRQ GNGENTVNLS LLLQRCNEVQ LWVATEILLC SQLGKRVQLV KKFIKIAAHC KAQQNLNSFF AIVMGLNTAS VSRLSQTWEK IPGKFKKLFS ELESLTDPSL NHKAYRDAFK KMKPPKIPFM PLLLKDVTFI HEGNKTFLDN LVNFEKLHMI ADTVRTLRHC RTNQFGSDVS PKEQQELKSY VNHLYVIDSQ QALFELSHRL EPRA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Andere Bezeichnung: Rapgef5 (RAPGEF5 Produkte)

Synonyme: GFR Protein, MR-GEF Protein, REPAC Protein, 4932413M22 Protein, C86120 Protein, D030051B22Rik Protein, Mrgef Protein, mKIAA0277 Protein, mmr-gef Protein, RGD1562891 Protein, rMR-GEF Protein, MGC154311 Protein, Rap guanine nucleotide exchange factor 5 Protein, Rap guanine nucleotide exchange factor (GEF) 5 Protein, Rap guanine nucleotide exchange factor 5 S homeolog Protein, RAPGEF5 Protein, Rapgef5 Protein, rapgef5.S Protein

Hintergrund: Rap guanine nucleotide exchange factor 5 (Guanine nucleotide exchange factor for Rap1) (M-Ras-regulated Rap GEF) (MR-GEF),FUNCTION: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity). {ECO:0000250}.

Molekulargewicht: 93.7 kDa

UniProt: Q8C0Q9

Pathways: RTK Signalweg, Neurotrophin Signalübertragung, Hepatitis C