SH3RF2 Protein (AA 1-735) (Strep Tag)

Produktnummer ABIN3125524

Produktdetails

Target: SH3RF2 (SH3 Domain Containing Ring Finger 2 (SH3RF2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-735

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SH3RF2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MDDLTLLDLL ECPVCFEKLD VTAKVLPCQH TFCKPCLQRI FKAHKELRCP ECRTLVFCSI EALPANLLLV RLLDGVRSGQ SSWKGGSFRR PRILTLQDNR KAKSSPRSLQ ASPFRLVPSV RIHMDGVPRA KALCNYRGKN PGDLKFNKGD VILLRRQLDE NWYQGEINGV SGIFPASSVE VIKQLPQPPP LCRALYNFDL RDKDKSENQD CLTFLKDDVI TVISRVDENW AEGKLGDKVG IFPILFVEPN VSARHLLENK GHQLSRTRHL SLMSSPSRGK ATNTSSLRKS PGSRRKGSGQ FSMTTALNTL NRTVHSPEGH QMVEISTPML ISSNSPSVLT QHGDKADFPT SSTGQVSSSQ PAPASPGHST AMVSVPSSQQ HLSNNMFVAL HTYSAHRPEE LDLQKGEGIR VLGKYQDGWL KGLSLLTGRT GIFPSDYVIP VFSSTARKTS SFPDSRSPTV CTTWALSTSS VSSQGSFAEG DPRQSGPFKS VFVPTAVVNP SRSTPGPGSS GQGSLRKVRS SMRKNGSLQR PVQSGIPTFM VGSLRCSPTM VIRPQRFQFY PPQGMTPSPT PIMVEMGSKS IYTGEPALTC INRGSKTRIH SAGNSIIMEG KETPIKSEPP PKPPASAPPS ILVKPENSKN GTEKQVKTVR FQNYSPPPTK HSASSPTSGK HDHPATLKGS QHEAVSSGGE MTILFAHRSG CHSGQQTDLR RKSAFGKTMP LLSTASATQT LFPSK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SH3RF2 (SH3 Domain Containing Ring Finger 2 (SH3RF2))

Andere Bezeichnung: Sh3rf2 (SH3RF2 Produkte)

Synonyme: MGC81405 Protein, SH3RF2 Protein, HEPP1 Protein, POSHER Protein, PPP1R39 Protein, RNF158 Protein, 2310046K19 Protein, 9130023G24Rik Protein, AI452083 Protein, Ppp1r39 Protein, Posh2 Protein, SH3 domain containing ring finger 2 Protein, SH3 domain containing ring finger 2 S homeolog Protein, SH3RF2 Protein, sh3rf2.S Protein, sh3rf2 Protein, Sh3rf2 Protein

Hintergrund: E3 ubiquitin-protein ligase SH3RF2 (EC 2.3.2.27) (Protein phosphatase 1 regulatory subunit 39) (RING finger protein 158) (RING-type E3 ubiquitin transferase SH3RF2) (SH3 domain-containing RING finger protein 2),FUNCTION: Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation. Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity (By similarity). {ECO:0000250|UniProtKB:Q8TEC5}.

Molekulargewicht: 80.1 kDa

UniProt: Q8BZT2