MOBKL1A Protein (AA 2-216) (His tag)

Produktnummer ABIN3125111

Produktdetails

Target: MOBKL1A (MOB1B) (MOB Kinase Activator 1B (MOB1B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-216

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MOBKL1A Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: SFLFGSRSSK TFKPKKNIPE GSHQYELLKH AEATLGSGNL RMAVMLPEGE DLNEWVAVNT VDFFNQINML YGTITDFCTE ESCPVMSAGP KYEYHWADGT NIKKPIKCSA PKYIDYLMTW VQDQLDDETL FPSKIGVPFP KNFMSVAKTI LKRLFRVYAH IYHQHFDPVI QLQEEAHLNT SFKHFIFFVQ EFNLIDRREL APLQELIEKL TSKDR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Mob1b Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MOBKL1A (MOB1B) (MOB Kinase Activator 1B (MOB1B))

Andere Bezeichnung: Mob1b (MOB1B Produkte)

Synonyme: MATS2 Protein, MOB4A Protein, MOBKL1A Protein, 1110003E08Rik Protein, AU015450 Protein, B230364F10 Protein, Mobkl1a Protein, mobkl1a Protein, mobkl1aa Protein, wu:fj65h03 Protein, zgc:56156 Protein, zgc:77872 Protein, MOB1B Protein, mob4a Protein, mats2 Protein, C2orf6 Protein, MOB1 Protein, MOBK1B Protein, Mob4B Protein, cb512 Protein, mobkl1ab Protein, sb:cb512 Protein, zgc:111840 Protein, MOB kinase activator 1B Protein, MOB kinase activator 1Ba Protein, Mps one binder kinase activator-like 1A Protein, mps one binder kinase activator-like 1A Protein, MOB kinase activator 1B L homeolog Protein, MOB kinase activator 1Bb Protein, MOB1B Protein, Mob1b Protein, mob1ba Protein, mol1a Protein, LOC100282811 Protein, LOC100285953 Protein, mob1b Protein, mob1b.L Protein, mob1bb Protein

Hintergrund: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L (By similarity). {ECO:0000250}.

Molekulargewicht: 25.9 kDa Including tag.

UniProt: Q8BPB0