IFT74 Protein (AA 1-600) (His tag)
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- Target Alle IFT74 Produkte
- IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-600
- Spezies
- Maus
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses IFT74 Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
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MASNHKSSAP RPISRGGIGL TGRPPSGIRP PSGNVRVATA MPPTTARPGS RGGPLGTGGV LSSQIKVADR PVTQQGLSGM KTGMKGPQRQ ILDKSYYLGL LRSKISELTT EINKLQKEIE MYNQENSVYL SYEKRAETLA VEIKDFQGQL ADYNMLVDKL NTNTEMEEVM SDYNMLKAQN DRETQSMDVI FTERQAKEKQ IRSVEEEVEQ EKQAADGIIK NMSPEKQVKY IEMKTTNEKL LQELDTLQQQ LDSLNMKKES LETEIAHSQV KQEAVLLYEK LYELESHRDQ MIAEDKSMGS PMEERERLLK QVKEDNQEIA SMERQLTDIK EKINQFSEEI RQLDMDLEEH QGEMNQKYKE LKKREENMDA FIETFEETKN QELERKAQIE ASIITLLEHC SRNINRMKQI SSITNQELKM MQDDLSFKST EMQKSQTTAR NLTSDSQRLQ LDLQKMELLE SKMTEEQQSL KNKIKQMTAD LETYSDLAAL KSSAEEKKKK LHQERTVLST HRNAFKKIME KLTSDYDTLK RQLQDNETHA QLTNLERKWQ HLEQNNFVMK EFIATKSQES DYQPVIKNVM KQIAEYNKTI MDALHNASRS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ift74 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Güteklasse
- Crystallography grade
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))
- Andere Bezeichnung
- Ift74 (IFT74 Produkte)
- Synonyme
- CCDC2 Protein, DKFZp469F1522 Protein, 1700029H06Rik Protein, Ccdc2 Protein, Cmg1 Protein, b2b796Clo Protein, CMG-1 Protein, CMG1 Protein, intraflagellar transport protein 74/72 Protein, intraflagellar transport 74 Protein, intraflagellar transport protein 74 homolog Protein, IFT74 Protein, ift74 Protein, LOC100632007 Protein, LOC100649180 Protein, Ift74 Protein
- Hintergrund
- Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis (By similarity). {ECO:0000250}.
- Molekulargewicht
- 70.3 kDa Including tag.
- UniProt
- Q8BKE9
- Pathways
- Hedgehog Signalweg, Chromatin Binding
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