MAPK15 Protein (AA 1-549) (Strep Tag)

Produktnummer ABIN3124451

Produktdetails

Target: MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-549

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses MAPK15 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MCAAEVDRHV AQRYLIKRRL GKGAYGIVWK AMDRRTGEVV AIKKIFDAFR DQIDAQRTFR EIMLLKEFGG HPNIIRLLDV IPAKNDRDIY LVFESMDTDL NAVIQKGRLL KDIHKRCIFY QLLRATKFIH SGRVIHRDQK PANVLLDSAC RVKLCDFGLA RSLGDLPEGP GGQALTEYVA TRWYRAPEVL LSSRWYTPGV DMWSLGCILG EMLRGQPLFP GTSTFHQLEL ILKTIPLPSM EELQDLGSDY SALILQNLGS RPQQTLDALL PPDTPPEALD LLKRLLAFAP DKRLSAEQAL QHPYVQRFHC PDREWARESD VRLPVHEGDQ LSAPEYRKRL YQIILEQSGN SRSPREEGLG VVASRAELRA SPARTQSLKS GVLPQVPAET PARKRGPKPP RSPGHDPEHV EVRRQSSDPL FQLPPPGRGE RPPGATGQPP SAPSGVKTQV RAMAPSLTSQ AEAQAANQAL IRSDPARGGG PRAVGARRVP SRLPREAPEP RPGRRMFGIS VSQGAQGAAR AALGGYSQAY GTVCRSALGR LPLLPGPRA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))

Andere Bezeichnung: Mapk15 (MAPK15 Produkte)

Synonyme: MGC138052 Protein, MGC99048 Protein, erk7 Protein, zgc:110345 Protein, ERK7 Protein, ERK8 Protein, BC048082 Protein, Erk7 Protein, ATMPK15 Protein, F25P22.9 Protein, F25P22_9 Protein, MAP kinase 15 Protein, mitogen-activated protein kinase 15 Protein, mitogen-activated protein kinase 15 L homeolog Protein, MAP kinase 15 Protein, MAPK15 Protein, mapk15.L Protein, mapk15 Protein, Tsp_00300 Protein, Mapk15 Protein, MPK15 Protein

Hintergrund: Mitogen-activated protein kinase 15 (MAP kinase 15) (MAPK 15) (EC 2.7.11.24) (Extracellular signal-regulated kinase 7) (ERK-7) (Extracellular signal-regulated kinase 8) (ERK-8),FUNCTION: Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (By similarity) (PubMed:25823377). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. Regulates DA transporter (DAT) activity and protein expression via activation of RhoA. In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (By similarity). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (By similarity). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (PubMed:23351492). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (By similarity). {ECO:0000250|UniProtKB:Q8TD08, ECO:0000250|UniProtKB:Q9Z2A6, ECO:0000269|PubMed:23351492, ECO:0000269|PubMed:25823377}.

Molekulargewicht: 60.7 kDa

UniProt: Q80Y86