CPEB3 Protein (AA 1-716) (Strep Tag)

Produktnummer ABIN3124063

Produktdetails

Target: CPEB3 (Cytoplasmic Polyadenylation Element Binding Protein 3 (CPEB3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-716

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses CPEB3 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MQDDLLMDKS KTQPQSQQQQ RQQQQQQQQL QPEPGAAEAP STPLSSEIPK PEDSSAVPAL SPASAPPAPN GPDKMQMESP LLPGLSFHQP PQQPPPPQEP TAPGASLSPS FGSTWSTGTT NAVEDSFFQG ITPVNGTMLF QNFPHHVNPV FGGTFSPQIG LAQTQHHQQP PPPAPQPPQP AQPPQAQPSQ QRRSPASPSQ APYAQRSAAA YGHQPIMTSK PSSSSAVAAA AAAAAASSAS SSWNTHQSVN AAWSAPSNPW GGLQAGRDPR RAVGVGVGVG VGVPSPLNPI SPLKKPFSSN VIAPPKFPRA APLTSKSWME DNAFRTDNGN NLLPFQDRSR PYDTFNLHSL ENSLMDMIRT DHEPLKGKHY PNSGPPMSFA DIMWRNHFAG RMGINFHHPG TDNIMALNTR SYGRRRGRSS LFPFEDAFLD DSHGDQALSS GLSSPTRCQN GERVERYSRK VFVGGLPPDI DEDEITASFR RFGPLVVDWP HKAESKSYFP PKGYAFLLFQ EESSVQALID ACLEEDGKLY LCVSSPTIKD KPVQIRPWNL SDSDFVMDGS QPLDPRKTIF VGGVPRPLRA VELAMIMDRL YGGVCYAGID TDPELKYPKG AGRVAFSNQQ SYIAAISARF VQLQHNDIDK RVEVKPYVLD DQMCDECQGT RCGGKFAPFF CANVTCLQYY CEYCWASIHS RAGREFHKPL VKEGGDRPRH VPFRWS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CPEB3 (Cytoplasmic Polyadenylation Element Binding Protein 3 (CPEB3))

Andere Bezeichnung: Cpeb3 (CPEB3 Produkte)

Synonyme: si:ch211-129m12.2 Protein, 4831444O18Rik Protein, mKIAA0940 Protein, RGD1564670 Protein, cytoplasmic polyadenylation element binding protein 3 Protein, cpeb3 Protein, CPEB3 Protein, LOAG_07772 Protein, Cpeb3 Protein

Hintergrund: Cytoplasmic polyadenylation element-binding protein 3 (CPE-BP3) (CPE-binding protein 3) (mCPEB-3),FUNCTION: Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator (PubMed:17024188, PubMed:26074072). In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3'-UTR, but binds to a U-rich loop within a stem-loop structure (PubMed:17024188). Required for the consolidation and maintenance of hippocampal-based long term memory (PubMed:26074003). In the basal state, binds to the mRNA 3'-UTR of the glutamate receptors GRIA1 and GRIA2 and negatively regulates their translation (PubMed:17024188, PubMed:22153079). Also represses the translation of DLG4, GRIN1 GRIN2A and GRIN2B (PubMed:24155305). When activated, acts as a translational activator of GRIA1 and GRIA2 (PubMed:22153079, PubMed:26074003). In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation (PubMed:26074071). Binds to the 3'-UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception (PubMed:26915043). Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation (PubMed:26074072). Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay (By similarity). In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance (By similarity). In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression (By similarity). {ECO:0000250|UniProtKB:Q8NE35, ECO:0000269|PubMed:17024188, ECO:0000269|PubMed:22153079, ECO:0000269|PubMed:22711986, ECO:0000269|PubMed:24155305, ECO:0000269|PubMed:26074003, ECO:0000269|PubMed:26074071, ECO:0000269|PubMed:26074072, ECO:0000269|PubMed:26915043}.

Molekulargewicht: 78.3 kDa

UniProt: Q7TN99