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ATP1A2 Protein (AA 151-286) (His tag)

Crystallography grade ATP1A2 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3123770
  • Target Alle ATP1A2 Proteine anzeigen
    ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 151-286
    Spezies
    • 6
    • 4
    • 2
    Maus
    Quelle
    • 4
    • 4
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses ATP1A2 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG TARGIVIATG DRTVMGRIAT LASGLEVGQT PIAMEI
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Atp1a2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ATP1A2 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))
    Andere Bezeichnung
    Atp1a2 (ATP1A2 Produkte)
    Synonyme
    C11orf29 Protein, TIF1D Protein, TIF1DELTA Protein, AW060654 Protein, Atpa-3 Protein, mKIAA0778 Protein, RATATPA2 Protein, FHM2 Protein, MHP2 Protein, atp1a2 Protein, atp[a]2 Protein, atpa2 Protein, cb702 Protein, fa10d04 Protein, fa10d05 Protein, wu:fa10d05 Protein, ATP2A Protein, atp1a2.L Protein, fhm2 Protein, mhp2 Protein, ATP1A2 Protein, ATP1A4 Protein, tripartite motif containing 66 Protein, ATPase, Na+/K+ transporting, alpha 2 polypeptide Protein, ATPase Na+/K+ transporting subunit alpha 2 Protein, ATPase, Na+/K+ transporting, alpha 2a polypeptide Protein, sodium/potassium-transporting ATPase subunit alpha-2 Protein, ATPase, Na+/K+ transporting, alpha 2 polypeptide S homeolog Protein, TRIM66 Protein, Atp1a2 Protein, ATP1A2 Protein, atp1a2a Protein, atp1a2.S Protein, LOC488636 Protein
    Hintergrund
    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). {ECO:0000250}.
    Molekulargewicht
    15.6 kDa Including tag.
    UniProt
    Q6PIE5
    Pathways
    Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process
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