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POLR2M Protein (AA 1-366) (His tag)

Crystallography grade GRINL1A Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3123621
  • Target Alle POLR2M (GRINL1A) Proteine anzeigen
    POLR2M (GRINL1A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate-Like 1A (GRINL1A))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-366
    Spezies
    • 5
    • 1
    • 1
    Maus
    Quelle
    • 2
    • 2
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses POLR2M Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MFSLPRGFEP PAPEDLGRQS SAELRERLRR QERLLRNEKF ICKLPDKGKK ISDTVAKLKA AISEREEVRG RSELFHPVSV DCKLRQKATT RADTDVDKAQ SSDLMLDTSS LDPDCSSIDI KSSKSTSETQ GPTHLTHRGN EETLEAGYTV NSSPAAHIRA RAPSSEVKEH LPQHSVSSQE EEISSSIDSL FITKLQKITI ADQSEPSEEN TSTENFPELQ SETPKKPHYM KVLEMRARNP VPPPHKFKTN VLPTQQSDSP SHCQRGQSPA SSEEQRRRAR QHLDDITAAR LLPLHHLPAQ LLSIEESLAL QREQKQNYEE MQAKLAAQKL AERLNIKMQS YNPEGESSGR YREVRDEADA QSSDEC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Polr2m Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product GRINL1A Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    POLR2M (GRINL1A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate-Like 1A (GRINL1A))
    Andere Bezeichnung
    Polr2m (GRINL1A Produkte)
    Synonyme
    GCOM1 Protein, GRINL1A Protein, Gdown Protein, Gdown1 Protein, AA407243 Protein, AA408799 Protein, AI303746 Protein, AW553238 Protein, D9Wsu138e Protein, Grinl1a Protein, mKIAA4185 Protein, Glurr Protein, RNA polymerase II subunit M Protein, polymerase (RNA) II (DNA directed) polypeptide M Protein, POLR2M Protein, Polr2m Protein
    Hintergrund
    Isoform 1 appears to be stable component of the Pol II(G) complex form of RNA polymerase II. Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in Mediator-dependent regulation of transcription activation. Isoform 1 acts in vitro as negative regulator of transcriptional activation, this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II (By similarity). {ECO:0000250}.
    Molekulargewicht
    42.2 kDa Including tag.
    UniProt
    Q6P6I6
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