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SWAP70 Protein (AA 1-585) (His tag)

Crystallography grade SWAP70 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3123439
  • Target Alle SWAP70 Proteine anzeigen
    SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-585
    Spezies
    Maus
    Quelle
    • 2
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses SWAP70 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MRGLKDELLK AIWHAFTALD LDRSGKVSKS QLKVLSHNLC TVLKVPHDPV ALEEHFRDDD EGPVSNQGYM PYLNKFILEK VQDNFDKIEF NRMCWTLCVK KNLTKSPLLI TEDDAFKVWV IFNFLSEDKY PLIIVPEEIE YLLKKLTEAM GGGWQQEQFE HYKINFDDNK DGLSAWELIE LIGNGQFSKG MDRQTVSMAI NEVFNELILD VLKQGYMMKK GHKRKNWTER WFVLKPNIIS YYVSEDLKDK KGDILLDENC CVESLPDKDG KKCLFLIKCF DKTFEISASD KKKKQEWIQA IYSTIHLLKL GSPPPHKEAR QRRKELRRKL LAEQEELERQ MKELQAANEN KQQELESVRK KLEEAASRAA DEEKKRLQTQ VELQTRFSTE LEREKLIRQQ MEEQVAQKSS ELEQYLQRVR ELEDMYLKLQ EALEDERQAR QDEETVRKLQ ARLLEEESSK RAELEKWHLE QQQAIQTTEA EKQELEQQRV MKEQALQEAM AQLEQLELER KQALEQYEGV KKKLEMATHM TKSWKDKVAH HEGLIRLIEP GSKNPHLITN WGPAAFTQAE LEEREKSWKE KKTTE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Swap70 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product SWAP70 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    SWAP70 (SWAP Switching B-Cell Complex 70kDa Subunit (SWAP70))
    Andere Bezeichnung
    Swap70 (SWAP70 Produkte)
    Synonyme
    SWAP-70 Protein, 70kDa Protein, AV235546 Protein, HSPC321 Protein, swap70b Protein, zgc:63599 Protein, switching B-cell complex subunit SWAP70 Protein, SWA-70 protein Protein, SWAP switching B-cell complex 70 Protein, SWAP switching B-cell complex 70kDa subunit Protein, switching B-cell complex subunit SWAP70a Protein, SWAP70 Protein, Swap70 Protein, swap70 Protein, swap70a Protein
    Hintergrund
    Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity). {ECO:0000250}.
    Molekulargewicht
    69.9 kDa Including tag.
    UniProt
    Q6A028
    Pathways
    Production of Molecular Mediator of Immune Response
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