RAB3IP Protein (AA 1-428) (Strep Tag)

Produktnummer ABIN3123398

Produktdetails

Target: RAB3IP (RAB3A Interacting Protein (Rabin3) (RAB3IP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-428

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses RAB3IP Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MANDPLEGFH EVNLASPTSP DLLGVCDPGT QEQTTSPSVI YRPHPSTLCA APLQANALDL SDLPTQPVYS SPRHFNCAEV SNISAHAPDP ASSVPSAVAS GLTKLTSRKD SCNAEREFLQ GATITEASAG NDDIFGLSTD SLSRLRSPSV LEVREKGYER LKEELAKAQR EAHKMVREAN VKQATAEKQL KEAQGKIDVL QAEVAALKTL VLSSSPTSPT QEPLAAAKTP FKRGHTRNKS TSSAMGGSHQ DLSVIQPIVK DCKEADLSLY NEFRSWKDEP TMDRTCPFLD KIYQEDIFPC LTFAKSELAS AVLEAVENNT LSIEPVGLQP IRFVKASAVE CGGPKKCALT GQSKPCRHRI RLGDSSCCYY ISPFCRYRIT SVCNFFTYIR YIQQGLVKQQ DVDQMFWEVM QLRKEMSLAK LGYFKEEL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RAB3IP (RAB3A Interacting Protein (Rabin3) (RAB3IP))

Andere Bezeichnung: Rab3ip (RAB3IP Produkte)

Synonyme: rabin3 Protein, gtpat12 Protein, MGC64583 Protein, MGC75737 Protein, MGC82752 Protein, zgc:110270 Protein, DKFZp469A0532 Protein, DKFZp469O1131 Protein, RABIN3 Protein, B230311A06 Protein, Gtpat12 Protein, Rabin3 Protein, RAB3A interacting protein L homeolog Protein, RAB3A interacting protein Protein, RAB3A interacting protein (rabin3) Protein, rab3ip.L Protein, rab3ip Protein, RAB3IP Protein, Rab3ip Protein

Hintergrund: Rab-3A-interacting protein (Rab3A-interacting protein) (Rabin-3) (SSX2-interacting protein),FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Part of the ciliary targeting complex containing Rab11, ASAP1, RAB3IP and RAB11FIP3 and ARF4 that promotes RAB3IP preciliary vesicle trafficking to mother centriole and ciliogenesis initiation. {ECO:0000250|UniProtKB:Q96QF0}.

Molekulargewicht: 47.1 kDa

UniProt: Q68EF0