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DBH Protein (AA 42-621) (His tag)

Crystallography grade DBH Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3123260
  • Target Alle DBH Proteine anzeigen
    DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 42-621
    Spezies
    • 8
    • 2
    Maus
    Quelle
    • 5
    • 2
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses DBH Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    RGSEPPESPF PYHIPLDPEG ILELSWNVSY VQEIIHFQLQ VQGLRAGVLF GMSDRGEMEN ADLIMLWTDG DRAYFADAWS DRKGQIHLDS QQDYQLLQAQ RTRDGLSLLF KRPFVTCDPK DYVIEDDTVH LVYGILEEPF QSLEAINTSG LHTGLQRVQL LKSEVPTPSM PEDVQTMDIR APDILIPDNE TTYWCYITEL PPRFPRHHII MYEAIVTEGN EALVHHMEVF QCAAESEDFP QFNGPCDSKM KPDRLNYCRH VLAAWALGAK AFYYPKEAGV PFGGPGSSPF LRLEVHYHNP RKIQGRQDSS GIRLHYTATL RRYDAGIMEL GLVYTPLMAI PPQETAFVLT GYCTDKCTQM ALQDSGIHIF ASQLHTHLTG RKVVTVLARD GQERKVVNRD NHYSPHFQEI RMLKKVVTVY PGDVLITSCT YNTENKTLAT VGGFGILEEM CVNYVHYYPQ TELELCKSAV DDGFLQKYFH MVNRFSSEEV CTCPQASVPQ QFSSVPWNSF NRDMLKALYD YAPISMHCNK TSAVRFPGEW NLQPLPKITS TLEEPTPRCP IRQTQSPANP TVPITTEADA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Dbh Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product DBH Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))
    Andere Bezeichnung
    Dbh (DBH Produkte)
    Synonyme
    CG1543 Protein, DBH Protein, Dbh Protein, Dmel\\CG1543 Protein, T beta h Protein, TBH Protein, TBetaH Protein, TBetah Protein, TBh Protein, TbetaH Protein, TbetaM Protein, Tbetah Protein, tbh Protein, DBM Protein, DOPBHY Protein, Tbh Protein, dopamine beta-hydroxylase (dopamine beta-monooxygenase) Protein, Tyramine beta hydroxylase Protein, MOXD1 homolog 2 Protein, dopamine beta hydroxylase Protein, dopamine beta-hydroxylase Protein, dbh Protein, Tbh Protein, LOC5574699 Protein, CpipJ_CPIJ004736 Protein, CpipJ_CPIJ004737 Protein, CpipJ_CPIJ014202 Protein, CpipJ_CPIJ016745 Protein, CpipJ_CPIJ019034 Protein, DBH Protein, Dbh Protein, LOC101110668 Protein
    Hintergrund
    Conversion of dopamine to noradrenaline. {ECO:0000269|PubMed:27148966, ECO:0000269|PubMed:7715704}.
    Molekulargewicht
    66.8 kDa Including tag.
    UniProt
    Q64237
    Pathways
    Carbohydrate Homeostasis
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