PHLDA1 Protein (AA 1-405) (His tag)
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- Target Alle PHLDA1 Proteine anzeigen
- PHLDA1 (Pleckstrin Homology-Like Domain, Family A, Member 1 (PHLDA1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-405
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Spezies
- Maus
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses PHLDA1 Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
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MRRTPAAERL SELGFPPRRG RQEPPFPLGV TRGWGGWPIE KRREGPRPVP FSERSPEDGR EQPAHGSGIL WRVRTRLSLC RDPEPPPPPP PLCLLRVSLL CALRAGGRGS RWGEDGAGLL LLPPAGASGS LKAERSSSTP YAGRMLENSG CKALKEGVLE KRSDGLLQLW KKKCCILTEE GLLLIPPKQL QQQQQQQQPG QGTAEPSQPS GPTVASLEPP VKLKELHFSN MKTVDCVERK GKYMYFTVVM TEGKEIDFRC PQDQGWNAEI TLQMVQYKNR QAILAVKSTR QKQQHLVQQQ PPQTQQIQPQ PQPQIQPQPQ PQIQPQPQPQ PQPQPQPQPQ PQPQQLHSYP HPHPHPYSHP HQHPHPHPHP HPHPHPHPYQ LQHAHQPLHS QPQGHRLLRS TSNSA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Phlda1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product PHLDA1 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- PHLDA1 (Pleckstrin Homology-Like Domain, Family A, Member 1 (PHLDA1))
- Andere Bezeichnung
- Phlda1 (PHLDA1 Produkte)
- Synonyme
- DT1P1B11 Protein, PHRIP Protein, TDAG51 Protein, Tdag Protein, pleckstrin homology like domain family A member 1 Protein, pleckstrin homology like domain, family A, member 1 Protein, pleckstrin homology-like domain, family A, member 1 Protein, pleckstrin homology-like domain, family A, member 1 L homeolog Protein, PHLDA1 Protein, Phlda1 Protein, phlda1.L Protein
- Hintergrund
- Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. Required for TCR-induced apoptosis and expression of TNFRSF6/FAS in a T-cell hybridoma cell line. May be involved in translational regulation. {ECO:0000269|PubMed:15037619, ECO:0000269|PubMed:8673705}.
- Molekulargewicht
- 46.5 kDa Including tag.
- UniProt
- Q62392
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