BPIFB1 Protein (AA 22-474) (His tag)

Produktnummer ABIN3122859

Produktdetails

Target: BPIFB1 (BPI Fold Containing Family B, Member 1 (BPIFB1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-474

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses BPIFB1 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: MNVYPPAVL NLGPEVIQKH LTQALKDHDA TAILQELPLL RAMQDKSGSI PILDSFVHTV LRYIIWMKVT SANILQLDVQ PSTYDQELVV RIPLDMVAGL NTPLIKTIVE FQMSTEVQAL IRVERSKSGP AHLNLSDCSS NESTLRLSLL HKLSFVVNSL AKNVMNLLVP ALPQIVKNHL CPVIQQAFDD MYEDFLRLTT APIALSPGAL EFGLLSPAIQ DSNILLNLKA KLLDSQARVT NWFNNSATSL METTPDRAPF SLTVRQDLVN AIVTTLVPKE ELVILLRFVI PDVARQLQMD IKEINAEAAN KLGPTQMLKI FTHSTPHIVL NEGSARAAQS VVLEVFPTNT DVRPFFSLGI EASYEAQFFT EDNRLMLNFN NVSIERIKLM ISDIKLFDPE VLKDTLTKIL EYTLLPNENG KLRTGVPMSM SKALGYEKAMW SVSKGALKLT PASSHHHHHH

Produktmerkmale:

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

C-terminal His-tag

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: (20 mM Hepes, pH 7.4; 50 mM NaCl; 0,1% Triton

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: BPIFB1 (BPI Fold Containing Family B, Member 1 (BPIFB1))

Andere Bezeichnung: Bpifb1 (BPIFB1 Produkte)

Synonyme: C20orf114 Protein, LPLUNC1 Protein, Lplunc1 Protein, C13H20orf114 Protein, RGD1563047 Protein, BPI fold containing family B member 1 Protein, BPI fold containing family B, member 1 Protein, BPIFB1 Protein, Bpifb1 Protein

Hintergrund: May play a role in innate immunity in mouth, nose and lungs. Binds bacterial lipopolysaccharide (LPS) and modulates the cellular responses to LPS. May be involved in formation of the left-right axis in the node of the developing embryo. {ECO:0000269|PubMed:14745963, ECO:0000269|PubMed:15028288}.

Molekulargewicht: 51.3 kDa Including tag.

UniProt: Q61114