DPH1 Protein (AA 1-438) (Strep Tag)

Produktnummer ABIN3122522

Produktdetails

Target: DPH1 (DPH1 Homolog (DPH1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-438

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses DPH1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAALVVSETA EPGSRVGPGR GRISRGRLAN QIPPEVLNNP QLQAAVQVLP SNYNFEIPKT IWRIQQAQAK KVALQMPEGL LLFACTIVDI LERFTEAEVM VMGDVTYGAC CVDDFTARAL GVDFLVHYGH SCLVPMDTSV QDFRVLYVFV DIRIDTAHLL DSVRLTFTPG SSLALVSTIQ FVSTLQAAAQ ELKADYHISV PQCKPLSPGE ILGCTSPRLS KEVEAVVYLG DGRFHLESVM IANPNIPAYR YDPYGKVLSR EYYDHQRMQA TRQEAIAAAR SAKSWGLILG TLGRQGSPKI LEHLESQLRN LGLPFVRLLL SEIFPSKLSL LPEVDVWVQV ACPRLSIDWG SAFPKPLLTP YEAAVALKDI SWQQPYPMDF YSGSSLGPWT VNYGRDRAPR GLCQPASDKV QQGSRGGSPA PACESCNCAD QKATSPAP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DPH1 (DPH1 Homolog (DPH1))

Andere Bezeichnung: Dph1 (DPH1 Produkte)

Synonyme: DPH1 Protein, DPH1-OVCA2 Protein, dph1-ovca2 Protein, dph2l Protein, dph2l1 Protein, ovca1 Protein, DPH2L Protein, DPH2L1 Protein, OVCA1 Protein, zgc:110702 Protein, 2310011M22Rik Protein, 4930488F09Rik Protein, AW551873 Protein, Dph2l1 Protein, Ovca1 Protein, RGD1562694 Protein, diphthamide biosynthesis 1 Protein, diphthamide biosynthesis protein 1 Protein, diphthamide biosynthesis 1 L homeolog Protein, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 Protein, DPH1 Protein, NCU08503 Protein, ATEG_01183 Protein, LELG_00973 Protein, HCAG_03806 Protein, AOR_1_728014 Protein, PTRG_05729 Protein, CTRG_01524 Protein, UREG_06411 Protein, BDBG_05574 Protein, MCYG_04396 Protein, PITG_12187 Protein, MGYG_02224 Protein, LOC100284663 Protein, dph1.L Protein, dph1 Protein, Dph1 Protein, dph-1 Protein

Hintergrund: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 (EC 2.5.1.108) (Diphthamide biosynthesis protein 1) (Diphtheria toxin resistance protein 1) (Ovarian cancer-associated gene 1 protein homolog) (S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1),FUNCTION: Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (PubMed:15485916, PubMed:24895408). DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase (By similarity). Acts as a tumor suppressor (PubMed:14744934, PubMed:15661533, PubMed:24895408). {ECO:0000250|UniProtKB:P40487, ECO:0000269|PubMed:14744934, ECO:0000269|PubMed:15485916, ECO:0000269|PubMed:15661533, ECO:0000269|PubMed:24895408}.

Molekulargewicht: 48.0 kDa

UniProt: Q5NCQ5