PEX7 Protein (AA 1-318) (Strep Tag)

Produktnummer ABIN3121272

Produktdetails

Target: PEX7 (Peroxisomal Biogenesis Factor 7 (PEX7))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-318

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses PEX7 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MSAARTLRVP GRHGYAAEFS PYLPGRLACA AAQHYGIAGC GTLLVLDQNE SGLQIFRSFD WNDGLFDVTW SENNEHVLVT CSGDGSLQLW DTAKATGPLQ VYKEHTQEVY SVDWSQTRGE QLVVSGSWDQ TVKVWDPTVG NSLCTFRGHE SVIYSTIWSP HIPGCFASAS GDQTLRIWDV KTTGVRIVIP AHQTEILSCD WCKYNENLVV TGAVDCSLRG WDLRNVRQPV FELLGHTYAI RRVKFSPFHA SVLASCSYDF TVRFWNFSKP DPLLETVEHH TEFTCGLDLS LQSPTQVADC SWDETIKIYD PVCLTVPG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PEX7 (Peroxisomal Biogenesis Factor 7 (PEX7))

Andere Bezeichnung: Pex7 (PEX7 Produkte)

Synonyme: PEX7 Protein, pex7 Protein, DDBDRAFT_0206295 Protein, DDBDRAFT_0233068 Protein, DDB_0206295 Protein, DDB_0233068 Protein, MmPEX7 Protein, PBD9B Protein, PTS2R Protein, RCDP1 Protein, RD Protein, zgc:103552 Protein, Peroxin-7 Protein, peroxisomal biogenesis factor 7 Protein, WD40 repeat-containing protein Protein, PEX7 Protein, pex7 Protein, Pex7 Protein

Hintergrund: Peroxisomal targeting signal 2 receptor (PTS2 receptor) (Peroxin-7),FUNCTION: Receptor required for the peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal (PubMed:9090381, PubMed:12915479). Specifically binds to cargo proteins containing a PTS2 peroxisomal targeting signal in the cytosol (By similarity). Cargo protein-binding triggers interaction with PEX5 and formation of a ternary complex composed of PEX5 and PEX7 along with PTS2-containing cargo proteins, which is tranlocated into peroxisomes by passing through the PEX13-PEX14 docking complex (By similarity). {ECO:0000250|UniProtKB:O00628, ECO:0000269|PubMed:12915479, ECO:0000269|PubMed:9090381}.

Molekulargewicht: 35.5 kDa

UniProt: P97865

Pathways: Monocarboxylic Acid Catabolic Process