PITX2 Protein (AA 1-317) (Strep Tag)

Produktnummer ABIN3121211

Produktdetails

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-317

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses PITX2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: METNCRKLVS ACVQLGVQPA AVECLFSKDS EIKKVEFTDS PKSRKESASS KLFPRQHPGA NEKDKGQQGK NEDVGAEDPS KKKRQRRQRT HFTSQQLQEL EATFQRNRYP DMSTREEIAV WTNLTEARVR VWFKNRRAKW RKRERNQQAE LCKNGFGPQF NGLMQPYDDM YPGYSYNNWA AKGLTSASLS TKSFPFFNSM NVNPLSSQSM FSPPNSISSM SMSSSMVPSA VTGVPGSSLN SLNNLNNLSS PSLNSAVPTP ACPYAPPTPP YVYRDTCNSS LASLRLKAKQ HSSFGYASVQ NPASNLSACQ YAVDRPV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Andere Bezeichnung: Pitx2 (PITX2 Produkte)

Synonyme: ARP1 Protein, Brx1 Protein, IDG2 Protein, IGDS Protein, IGDS2 Protein, IHG2 Protein, IRID2 Protein, Otlx2 Protein, PTX2 Protein, RGS Protein, RIEG Protein, RIEG1 Protein, RS Protein, 9430085M16Rik Protein, Brx1a Protein, Brx1b Protein, Munc30 Protein, Pitx2a Protein, Pitx2b Protein, Pitx2c Protein, Pitx2cbeta Protein, Ptx2 Protein, Rieg Protein, pitx2a Protein, pitx2c Protein, zgc:110508 Protein, XPtx2b Protein, xPtx2 Protein, xpitx2 Protein, arp1 Protein, brx1 Protein, idg2 Protein, igds Protein, igds2 Protein, ihg2 Protein, irid2 Protein, otlx2 Protein, pitx2-A Protein, ptx2 Protein, rgs Protein, rieg Protein, rieg1 Protein, pitx-2 Protein, pitx2 Protein, paired like homeodomain 2 Protein, paired-like homeodomain transcription factor 2 Protein, paired-like homeodomain 2 Protein, paired like homeodomain 2 S homeolog Protein, paired like homeodomain 2 L homeolog Protein, PITX2 Protein, Pitx2 Protein, pitx2 Protein, pitx2.S Protein, pitx2.L Protein

Hintergrund: Pituitary homeobox 2 (ALL1-responsive protein ARP1) (BRX1 homeoprotein) (Bicoid-related homeobox protein 1) (Homeobox protein PITX2) (Orthodenticle-like homeobox 2) (Paired-like homeodomain transcription factor 2) (Solurshin),FUNCTION: May play a role in myoblast differentiation. When unphosphorylated, associates with an ELAVL1-containing complex, which stabilizes cyclin mRNA and ensuring cell proliferation. Phosphorylation by AKT2 impairs this association, leading to CCND1 mRNA destabilization and progression towards differentiation. {ECO:0000269|PubMed:20019746}., FUNCTION: [Isoform Ptx2c]: Involved in the establishment of left-right asymmetry in the developing embryo. {ECO:0000269|PubMed:10585561}.

Molekulargewicht: 35.3 kDa

UniProt: P97474

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development