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UBE2I Protein (AA 2-158) (His tag)

Crystallography grade UBE2I Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Produktnummer ABIN3120867
  • Target Alle UBE2I Proteine anzeigen
    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-158
    Spezies
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 7
    • 6
    • 4
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses UBE2I Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
    Sequenz
    SGIALSRLAQ ERKAWRKDHP FGFVAVPTKN PDGTMNLMNW ECAIPGKKGT PWEGGLFKLR MLFKDDYPSS PPKCKFEPPL FHPNVYPSGT VCLSILEEDK DWRPAITIKQ ILLGIQELLN EPNIQDPAQA EAYTIYCQNR VEYEKRVRAQ AKKFAPS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ube2i Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product UBE2I Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
    Andere Bezeichnung
    Ube2i (UBE2I Produkte)
    Synonyme
    C358B7.1 Protein, P18 Protein, UBC9 Protein, ubc9 Protein, ube2ia Protein, zUbc9 Protein, 5830467E05Rik Protein, F830028O17Rik Protein, Ubce2i Protein, Ubce9 Protein, UbcE2A Protein, ubce9 Protein, T13J8.70 Protein, T13J8_70 Protein, UBIQUITIN-PROTEIN LIGASE Protein, ubiquitin conjugating enzyme 9 Protein, ubiquitin conjugating enzyme E2 I Protein, ubiquitin-conjugating enzyme E2Ib Protein, ubiquitin-conjugating enzyme E2L Protein, ubiquitin-conjugating enzyme E2I Protein, ubiquitin conjugating enzyme E2I S homeolog Protein, ubiquitin conjugating enzyme 9 Protein, SUMO-conjugating enzyme UBC9 Protein, UBE2I Protein, ube2ib Protein, UBE2L Protein, Ube2i Protein, ube2i.S Protein, UBC9 Protein, ubc-9 Protein, LOC108703134 Protein
    Hintergrund
    Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5 (By similarity). Sumoylates p53/TP53 at 'Lys-386'. {ECO:0000250|UniProtKB:P63279, ECO:0000269|PubMed:16326389, ECO:0000269|PubMed:17187077, ECO:0000269|PubMed:22214662}.
    Molekulargewicht
    18.8 kDa Including tag.
    UniProt
    P63280
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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