STK39 Protein (AA 1-556) (Strep Tag)

Produktnummer ABIN3120322

Produktdetails

Target: STK39 (serine threonine Kinase 39 (STK39))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-556

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses STK39 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MAEPSGSPVH VQLSQQAAPV TAAAATAPAA ATSAPAPAPA PAPAASAAPA PAPAAAPAPA PAAQAVGWPI CRDAYELQEV IGSGATAVVQ AALCKPRQER VAIKRINLEK CQTSMDELLK EIQAMSQCSH PNVVTYYTSF VVKDELWLVM KLLSGGSMLD IIKYIVNRGE HKNGVLEEAI IATILKEVLE GLDYLHRNGQ IHRDLKAGNI LLGEDGSVQI ADFGVSAFLA TGGDVTRNKV RKTFVGTPCW MAPEVMEQVR GYDFKADMWS FGITAIELAT GAAPYHKYPP MKVLMLTLQN DPPTLETGVE DKEMMKKYGK SFRKLLSLCL QKDPSKRPTA AELLKCKFFQ KAKNREYLIE KLLTRTPDIA QRAKKVRRVP GSSGHLHKTE DGDWEWSDDE MDEKSEEGKA AASQEKSRRV KEENSEISVN AGGIPEQIQS LSVHDSQAQP NANEDYREGP CAVNLVLRLR NSRKELNDIR FEFTPGRDTA DGVSQELFSA GLVDGHDVVI VAANLQKIVD DPKALKTLTF KLASGCDGSE IPDEVKLIGF AQLSVS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: STK39 (serine threonine Kinase 39 (STK39))

Andere Bezeichnung: Stk39 (STK39 Produkte)

Synonyme: dcht Protein, pask Protein, spak Protein, MGC69537 Protein, DCHT Protein, PASK Protein, SPAK Protein, Spak Protein, AW227544 Protein, AW556857 Protein, RF005 Protein, Rnl5 Protein, serine/threonine kinase 39 Protein, serine/threonine kinase 39 L homeolog Protein, serine threonine kinase 39 Protein, stk39 Protein, STK39 Protein, stk39.L Protein, Stk39 Protein

Hintergrund: STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20-related kinase) (EC 2.7.11.1) (Serine/threonine-protein kinase 39),FUNCTION: Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed:16382158, PubMed:17488636, PubMed:19633012, PubMed:21486947). Specifically recognizes and binds proteins with a RFXV motif (PubMed:14563843). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed:14563843, PubMed:16382158, PubMed:17488636, PubMed:19633012, PubMed:21486947). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (By similarity). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx, simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (By similarity). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed:17488636, PubMed:19633012, PubMed:21486947). Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities (PubMed:21317537, PubMed:23542070). Phosphorylates RELT (PubMed:16530727). {ECO:0000250|UniProtKB:Q9UEW8, ECO:0000269|PubMed:14563843, ECO:0000269|PubMed:16382158, ECO:0000269|PubMed:16530727, ECO:0000269|PubMed:17488636, ECO:0000269|PubMed:19633012, ECO:0000269|PubMed:21317537, ECO:0000269|PubMed:21486947, ECO:0000269|PubMed:23542070}.

Molekulargewicht: 60.3 kDa

UniProt: Q9Z1W9