CDC7 Protein (AA 1-564) (Strep Tag)

Produktnummer ABIN3120202

Produktdetails

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-564

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses CDC7 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequenz: MEEPMAFSSL RGSDRCPADD SLKKYEQSVK LSGIKRDIEE LCEAVPQLVN VFKIKDKIGE GTFSSVYLAT AQLQEGHEEK IALKHLIPTS HPMRIAAELQ CLTVAGGQDN VMGLKYCFRK NDHVVIAMPY LEHESFLDIL NSLSFQEVRE YMYNLFVALK RIHQFGIVHR DVKPSNFLYN RRLKKYALVD FGLAQGTRDT KIELLKFVQS EAQQEDCSRN KYHGVVGHKG LLSRPAPKTV DQQCTPKTSV KRSYTQVHIK QGKDGKERSV GLSVQRSVFG ERNFNIHSSI SHESPAEKLI KQSKTVDIIS RKLATKKTAI STKAMNSVMR ETARSCPAVL TCDCYGSDRV CSVCLSRRQQ VAPRAGTPGF RAPEVLTKCP DQTTAIDMWS AGVIFLSLLS GRYPFYKASD DLTALAQIMT IRGSRETIQA AKAFGKSVLC SKEVPAQDLR ALCERLRGLD STTPRSASGP PGNASYDPAA SKNTDHKASR VQAAQAQHSE DSLYKRDNDG YWSHPKDCTS NSEGWDSVPD EAYDLLDKLL DLNPASRITA EAALLHAFFK DMCS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Andere Bezeichnung: Cdc7 (CDC7 Produkte)

Synonyme: CDC7L1 Protein, HsCDC7 Protein, Hsk1 Protein, huCDC7 Protein, AI597260 Protein, Cdc7l1 Protein, muCdc7 Protein, cell division cycle 7 Protein, cell division cycle 7-related protein kinase Protein, cell division cycle 7 (S. cerevisiae) Protein, CDC7 Protein, Tsp_08585 Protein, Cdc7 Protein

Hintergrund: Cell division cycle 7-related protein kinase (CDC7-related kinase) (muCdc7) (EC 2.7.11.1),FUNCTION: Kinase involved in initiation of DNA replication. Phosphorylates critical substrates that regulate the G1/S phase transition and initiation of DNA replication, such as MCM proteins and CLASPIN. {ECO:0000250|UniProtKB:O00311}.

Molekulargewicht: 62.8 kDa

UniProt: Q9Z0H0

Pathways: Mitotic G1-G1/S Phases, DNA Replication