TAF7 Protein (AA 1-341) (Strep Tag)

Produktnummer ABIN3119932

Produktdetails

Target: TAF7 (TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-341

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TAF7 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MSKNKDDAPH ELESQFILRL PPEYAATVRR AVQSGHVNLK DKLSIELHPD GRHGIVRVDR VPLAAKLVDL PCVTESLKTI DKKTFYKTAD ISQMLVATVD GDLYPPVEEA AATADPKANK KKDKDKEKKF VWNHGITLPL KNVRKRRFRK TAKKKYIESP DVEKEVKRLL STDAEAVSTR WEIIAEDETK ETENQGLDIS SPGMSGHRQG HDSLEHDELR EIFNDLSSSS EDEEDVNILD TEEDLERQLQ DKLNESDEQH QENEGTNQLV MGIQKQIDNM KGKLQETQDR AKRQEDLIMK VENLALKNRF QAVLDELKQK EDREKEQLSS LQEELESLLE K
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TAF7 (TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7))

Andere Bezeichnung: Taf7 (TAF7 Produkte)

Synonyme: CG2670 Protein, Dmel\\CG2670 Protein, E Protein, TAF Protein, TAF55 Protein, TAF7 Protein, TAF[[II]] Protein, TAF[[II]]55 Protein, TFIID Protein, Taf55 Protein, anon-84Ec Protein, dTAF[[II]]55 Protein, AO090012000400 Protein, CHUNP6888 Protein, 55kDa Protein, AI607308 Protein, BB007175 Protein, TAFII55 Protein, Taf2f Protein, TAF2F Protein, taf2f Protein, TBP-associated factor 7 Protein, transcription initiation factor tfiid 55 kDa subunit Protein, transcription initiation factor TFIID subunit D6 Protein, transcription initiation factor TFIID subunit 7 Protein, TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor Protein, TATA-box binding protein associated factor 7 Protein, TATA-box binding protein associated factor 7 L homeolog Protein, Taf7 Protein, CC1G_01188 Protein, PGTG_01179 Protein, AOR_1_682194 Protein, taf7 Protein, TAF7 Protein, taf7.L Protein

Hintergrund: Transcription initiation factor TFIID subunit 7 (RNA polymerase II TBP-associated factor subunit F) (Transcription initiation factor TFIID 55 kDa subunit) (TAF(II)55) (TAFII-55) (TAFII55),FUNCTION: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF7 forms a promoter DNA binding subcomplex of TFIID, together with TAF1 and TAF2. Part of a TFIID complex containing TAF10 (TFIID alpha) and a TFIID complex lacking TAF10 (TFIID beta). {ECO:0000250|UniProtKB:Q15545, ECO:0000269|PubMed:10438527}.

Molekulargewicht: 39.1 kDa

UniProt: Q9R1C0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway