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Adenylosuccinate Lyase Protein (ADSL) (AA 2-484) (His tag)

Crystallography grade ADSL Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3119817
  • Target Alle Adenylosuccinate Lyase (ADSL) Proteine anzeigen
    Adenylosuccinate Lyase (ADSL)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-484
    Spezies
    • 6
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 3
    • 3
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses Adenylosuccinate Lyase Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    AASGDPGSAE SYRSPLAARY ASREMCFLFS DRYKFQTWRQ LWLWLAEAEQ TLGLPITDEQ IQEMKSNLNN IDFQMAAEEE KRLRHDVMAH VHTFGHCCPK AAGIIHLGAT SCYVGDNTDL IILRNAFDLL LPKLARVISR LADFAKDRAD LPTLGFTHFQ PAQLTTVGKR CCLWIQDLCM DLQNLKRVRD ELRFRGVKGT TGTQASFLQL FEGDHQKVEQ LDKMVTEKAG FKRAFIITGQ TYTRKVDIEV LSVLASLGAS VHKICTDIRL LANLKEMEEP FEKQQIGSSA MPYKRNPMRS ERCCSLARHL MALTMDPLQT ASVQWFERTL DDSANRRICL AEAFLTADTI LNTLQNISEG LVVYPKVIER RIRQELPFMA TENIIMAMVK AGGSRQDCHE KIRVLSQQAA AVVKQEGGDN DLIERIRADA YFSPIHSQLE HLLDPSSFTG RAPQQVHRFL EEEVRPLLKP YGNEMAVKAE LCL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Adsl Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ADSL Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Adenylosuccinate Lyase (ADSL)
    Andere Bezeichnung
    Adsl (ADSL Produkte)
    Synonyme
    fi60b04 Protein, zgc:56061 Protein, wu:fi60b04 Protein, MGC53383 Protein, MGC69239 Protein, adsL Protein, F15C21.8 Protein, F15C21_8 Protein, AMPS Protein, ASASE Protein, ASL Protein, Adl Protein, Asl Protein, purB Protein, adenylosuccinate lyase Protein, adenylosuccinate lyase L homeolog Protein, adenylosuccinase; ASL Protein, Adenylosuccinate lyase Protein, L-Aspartase-like family protein Protein, ADSL Protein, adsl Protein, adsl.L Protein, ASL Protein, purB Protein, purB,adsL Protein, Mrub_2293 Protein, MMAH_RS02975 Protein, Arnit_0089 Protein, Mesil_1058 Protein, Slip_0597 Protein, Trad_2452 Protein, Deba_0652 Protein, Toce_1174 Protein, Acear_2324 Protein, Igag_0272 Protein, Saut_2156 Protein, Fbal_1996 Protein, Ndas_2434 Protein, Ilyop_0196 Protein, Lbys_2961 Protein, MFER_RS02920 Protein, Palpr_2192 Protein, Calni_0061 Protein, Ftrac_3746 Protein, AT1G36280 Protein, Olsu_1764 Protein, Adsl Protein
    Hintergrund
    Catalyzes two non-sequential steps in de novo AMP synthesis: converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate. {ECO:0000250|UniProtKB:P30566}.
    Molekulargewicht
    55.7 kDa Including tag.
    UniProt
    P54822
    Pathways
    Ribonucleoside Biosynthetic Process
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