RRBP1 Protein (AA 1-1410) (Strep Tag)

Produktnummer ABIN3118773

Produktdetails

Target: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1410

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses RRBP1 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MDIYDTQTLG VVVFGGFMVV SAIGIFLVST FSMKETSYEE ALANQRKEMA KTHHQKVEKK KKEKTVEKKG KTKKKEEKPN GKIPDHDPAP NVTVLLREPV RAPAVAVAPT PVQPPIIVAP VATVPAMPQE KLASSPKDKK KKEKKVAKVE PAVSSVVNSI QVLTSKAAIL ETAPKEVPMV VVPPVGAKGN TPATGTTQGK KAEGTQNQSK KAEGAPNQGR KAEGTPNQGK KTEGTPNQGK KAEGTPNQGK KAEGTPNQGK KAEGAQNQGK KVDTTPNQGK KVEGAPTQGR KAEGAQNQAK KVEGAQNQGK KAEGAQNQGK KGEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KVEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGK KVEGAQNQGK KAEGAQNQGK KAEGAQNQGK KAEGAQNQGQ KGEGAQNQGK KTEGAQGKKA ERSPNQGKKG EGAPIQGKKA DSVANQGTKV EGITNQGKKA EGSPSEGKKA EGSPNQGKKA DAAANQGKKT ESASVQGRNT DVAQSPEAPK QEAPAKKKSG SKKKGEPGPP DADGPLYLPY KTLVSTVGSM VFNEGEAQRL IEILSEKAGI IQDTWHKATQ KGDPVAILKR QLEEKEKLLA TEQEDAAVAK SKLRELNKEM AAEKAKAAAG EAKVKKQLVA REQEITAVQA RMQASYREHV KEVQQLQGKI RTLQEQLENG PNTQLARLQQ ENSILRDALN QATSQVESKQ NAELAKLRQE LSKVSKELVE KSEAVRQDEQ QRKALEAKAA AFEKQVLQLQ ASHRESEEAL QKRLDEVSRE LCHTQSSHAS LRADAEKAQE QQQQMAELHS KLQSSEAEVR SKCEELSGLH GQLQEARAEN SQLTERIRSI EALLEAGQAR DAQDVQASQA EADQQQTRLK ELESQVSGLE KEAIELREAV EQQKVKNNDL REKNWKAMEA LATAEQACKE KLLSLTQAKE ESEKQLCLIE AQTMEALLAL LPELSVLAQQ NYTEWLQDLK EKGPTLLKHP PAPAEPSSDL ASKLREAEET QSTLQAECDQ YRSILAETEG MLRDLQKSVE EEEQVWRAKV GAAEEELQKS RVTVKHLEEI VEKLKGELES SDQVREHTSH LEAELEKHMA AASAECQNYA KEVAGLRQLL LESQSQLDAA KSEAQKQSDE LALVRQQLSE MKSHVEDGDI AGAPASSPEA PPAEQDPVQL KTQLEWTEAI LEDEQTQRQK LTAEFEEAQT SACRLQEELE KLRTAGPLES SETEEASQLK ERLEKEKKLT SDLGRAATRL QELLKTTQEQ LAREKDTVKK LQEQLEKAED GSSSKEGTSV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Andere Bezeichnung: RRBP1 (RRBP1 Produkte)

Synonyme: 1700087N07Rik Protein, 5730465C04Rik Protein, ES/130 Protein, mKIAA1398 Protein, mRRp0 Protein, mRRp1.8 Protein, mRRp10 Protein, mRRp15a Protein, mRRp15b Protein, mRRp16.8 Protein, mRRp2 Protein, mRRp41 Protein, mRRp47 Protein, mRRp5.4 Protein, p180 Protein, ES130 Protein, RRp Protein, hES Protein, P180 Protein, rrbp1 Protein, sb:cb489 Protein, wu:fc47a01 Protein, ribosome binding protein 1 Protein, ribosome binding protein 1 homolog 180kDa (dog) Protein, ribosome binding protein 1b Protein, Rrbp1 Protein, RRBP1 Protein, rrbp1b Protein

Hintergrund: Ribosome-binding protein 1 (180 kDa ribosome receptor homolog) (RRp) (ES/130-related protein) (Ribosome receptor protein),FUNCTION: Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. {ECO:0000250}.

Molekulargewicht: 152.5 kDa

UniProt: Q9P2E9