PCDH10 Protein (AA 19-1040) (rho-1D4 tag)

Produktnummer ABIN3118771

Produktdetails

Target: PCDH10 (Protocadherin 10 (PCDH10))

Protein-Typ: Recombinant

Proteineigenschaft: AA 19-1040

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PCDH10 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: QLHYTVQEEQ EHGTFVGNIA EDLGLDITKL SARGFQTVPN SRTPYLDLNL ETGVLYVNEK IDREQICKQS PSCVLHLEVF LENPLELFQV EIEVLDINDN PPSFPEPDLT VEISESATPG TRFPLESAFD PDVGTNSLRD YEITPNSYFS LDVQTQGDGN RFAELVLEKP LDREQQAVHR YVLTAVDGGG GGGVGEGGGG GGGAGLPPQQ QRTGTALLTI RVLDSNDNVP AFDQPVYTVS LPENSPPGTL VIQLNATDPD EGQNGEVVYS FSSHISPRAR ELFGLSPRTG RLEVSGELDY EESPVYQVYV QAKDLGPNAV PAHCKVLVRV LDANDNAPEI SFSTVKEAVS EGAAPGTVVA LFSVTDRDSE ENGQVQCELL GDVPFRLKSS FKNYYTIVTE APLDREAGDS YTLTVVARDR GEPALSTSKS IQVQVSDVND NAPRFSQPVY DVYVTENNVP GAYIYAVSAT DRDEGANAQL AYSILECQIQ GMSVFTYVSI NSENGYLYAL RSFDYEQLKD FSFQVEARDA GSPQALAGNA TVNILIVDQN DNAPAIVAPL PGRNGTPARE VLPRSAEPGY LLTRVAAVDA DDGENARLTY SIVRGNEMNL FRMDWRTGEL RTARRVPAKR DPQRPYELVI EVRDHGQPPL SSTATLVVQL VDGAVEPQGG GGSGGGGSGE HQRPSRSGGG ETSLDLTLIL IIALGSVSFI FLLAMIVLAV RCQKEKKLNI YTCLASDCCL CCCCCGGGGS TCCGRQARAR KKKLSKSDIM LVQSSNVPSN PAQVPIEESG GFGSHHHNQN YCYQVCLTPE SAKTDLMFLK PCSPSRSTDT EHNPCGAIVT GYTDQQPDII SNGSILSNET KHQRAELSYL VDRPRRVNSS AFQEADIVSS KDSGHGDSEQ GDSDHDATNR AQSAGMDLFS NCTEECKALG HSDRCWMPSF VPSDGRQAAD YRSNLHVPGM DSVPDTEVFE TPEAQPGAER SFSTFGKEKA LHSTLERKEL DGLLTNTRAP YKPPYLTRKR IC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PCDH10 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PCDH10 (Protocadherin 10 (PCDH10))

Andere Bezeichnung: PCDH10 (PCDH10 Produkte)

Synonyme: OL-PCDH Protein, PCDH19 Protein, PCDH10 Protein, OLpcad Protein, cadherin Protein, 6430521D13Rik Protein, 6430703F07Rik Protein, OL-pc Protein, mKIAA1400 Protein, RGD1565811 Protein, ZPCR1 Protein, fc56b06 Protein, pcdh10 Protein, pcr1 Protein, wu:fc56b06 Protein, wu:fq40g12 Protein, wu:fq16b02 Protein, protocadherin 10 Protein, protocadherin 10a Protein, protocadherin 10b Protein, PCDH10 Protein, Pcdh10 Protein, pcdh10a Protein, pcdh10b Protein

Hintergrund: Potential calcium-dependent cell-adhesion protein.

Molekulargewicht: 112.0 kDa Including tag.

UniProt: Q9P2E7